Adipogen/SUMO Conjugation Reaction Buffer (10X)/AG-10T-0016-L005/5 ml
More Information Product Details
Product Type | Chemical |
Properties
Formulation | Liquid. |
Crossreactivity | Multi-species |
Declaration | Manufactured by Boston Biochem |
Other Product Data | Use: SUMO reaction buffer contains the optimal formulation for the use in conjugation assays of the ubiquitin-like modifiers, SUMO1, SUMO2, and SUMO3, to protein substrates in vitro. These SUMO proteins are conjugated to a variety of proteins in the presence of Ubc9 and SAE1/SAE2 (human). This buffer can be a supplement to SUMO conjugation kits. Dilute to 1X in final reaction. |
Shipping and Handling
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Aliquot to avoid freeze/thaw cycles. |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents
MSDS ![](\"https://www.ebiomall.com/images/adipogen/download.png\") Download PDF |
Product Specification Sheet
Datasheet | Download PDF |
Small ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. There are four known SUMOs (SUMO1-4). All SUMO proteins share a conserved ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following cleavage of a C-terminal prosegment, the C-terminal glycine residue of SUMO is enzymatically attached to a lysine residue on a target protein. In humans, SUMO is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme. In yeast, the SUMO-activating (E1) enzyme is Aos1/Uba2p. SUMOylation can occur without the requirement of a specific SUMO ligase (E3), where SUMO is transferred directly from UBE2I/Ubc9 to specific substrates. Unlike SUMO1, which is usually conjugated to proteins as a monomer, SUMO2 and SUMO3 are known to form high molecular weight polymers on proteins. SUMO precursor processing and deconjugation are catalyzed by a family of cysteine proteases known as SUMO-specific proteases (SENPs) and DeSUMOylating Isopeptidase 1.
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