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GenWay/Fluorogenic HDAC class 2a substrate/GWB-2F8206/250nmole
2026-08-23
Description:
Description: A fluorogenic, acetylated peptide substrate for HDAC4,5, 7 and 9. PURITY :>95% by HPLC. APPLICATIONS : Study enzyme kinetics, and screen small molecule inhibitors of HDAC class2a for drug discovery and HTS applications. SUPPLIED AS : DMSO solution QUANTITY : 50 µl @ 5 mM STORAGE : -20°C or -70°C. Assay Protocol: Material : Assay buffer, BPS catalog number 50031; Assay developer, BPS catalog number 50030; HDAC Substrate, catalog number 50040, Step 1 : Adding all reaction mixture to a low binding NUNC black plate, VWR catalog number 62408-936, 5 ul of HDAC4, 0.01 ug/ul, 35 ul of HDAC assay buffer, BPS catalog number 50031, 5 ul of 1 mg/ml BSA 5 ul of 200 uM substrate, BPS catalog number 50040, Incubate at 37 ° C for 30 min. Step 2 : Stop the reaction Add 50 ul of HDAC assay developer, 2x, BPS catalog number 50030, and incubate the plate at room temperature for 15 min Step 3 : Read sample in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range 350-380 nm and detection of emitted light in the range 440-460 nm. Format: DMSO solutionFormula: 5 mM solution in DMSOScientific Category: Deacetylase/SubstrateAdditional Information:
| Fluorogenic HDAC class 2a substrate |
| histone deacetylase, HDAC |
| 5 mM solution in DMSO |
| Dmso Solution |
| -20°C or -80°C |
| Research Use Only |
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