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Please click here for MSDS PDF DatasheetProduct Name: Chagas (Trypanosoma cruzi) IgG – ELISA# of Samples: 1 x 96 AssaysIntended Use: The Chagas (Trypanosoma cruzi) IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Trypanosoma cruzi in human serum or plasma (citrate). Introduction: Chagas’ disease occurs in Middle and South America. It is an infectious disease transmitted to humans through the bite wound caused by an infected bug of the family reduviidae. The infection is caused by Trypanosoma cruzi a unicellular parasite. The infection passes through different phases and causes an often chronic disease. 4 to 5 million people in South America are suffering from it. People living under poor conditions are mainly jeopardized. Up to 10 % of all infections are fatal; babies and neonates have a special risk.The infection is transmitted to humans by different bug species of the family reduviidae. The parasite does not enter the human body with the bit of the bug. Trypanosoma cruzi lives in faeces of the bug and invades into humans by skin wounds. Newborns are at risk by infection in utero. An infection via blood transfusion is also possible.1-2% of all infected people show symptoms after an incubation time of one to four weeks. Children up to 15 years old are mainly affected. The disease passes through three different phases.During acute phase fever, diarrhoea, gripes, swollen lymph nodes and swelling of the whole body appears. Especially in neonates and infants inflammation of heart or brain are possible. The acute phase lasts for ca. 4 weeks. The acute Chagas disease is mostly a disease of children. In many cases the disease is healed up.The following latency period is characterized by lack of symptoms for most of the patients. Occasionally a weakening of the immune system takes place. This phase can last several years.10 to 20 % of infected people reach the chronic phase. Different inner organs like heart, intestinal tract or neuronal system can be affected. Patients often die be sudden cardiac death or as the result of a chronic heart insufficiency. The prognosis depends upon the degree of heart variance.A variety of diagnostic methods have been used, but detection of antibodies to T. cruzi antigens remains the strongest method to diagnose infection.Principles of the assay: The qualitative immunoenzymatic determination of IgG-class antibodies against Chagas (Trypanosoma cruzi) is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.Microtiter strip wells are precoated with Trypanosoma cruzi antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Trypanosoma cruzi-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data.In immunocompromised patients and newborns serological data only have restricted value.Cross reaction of the antigens with antibodies against Leishmania, Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense cannot be excluded.References SOARES, M.B.P., PONTES-DE-CARAVALHO, L. AND RIBEIRO-DOS- SANTOS, R. (2001) THE PATHOGENESIS OF CHAGAS´DISEASE: WHEN AUTOIMMUNE AND PARASITE-SPECIFIC IMMUNE RESPONSES MEET. AN. ACAD. BRAS. CIENC. 73, 547-559FERREIRA, M.S. AND BORGES, A.S. (2002) SOME ASPECTS OF PROTOZOAN INFECTIONS IN IMMUNOCOMPROMISED PATIENTS- A REVIEW. MEM. INST. OSWALDO CRUZ, RIO DE JANEIRO 97, 443-457AÑEZ, N., CRISTANTE, G. AND ROJAS, A. (2004) UPDATE ON CHAGAS DISEASE IN VENEZUELA - A REVIEW. MEM. INST. OSWALDO CRUZ, RIO DE JANEIRO 99, 781-787MACEDO, A.M., MACHADO, C.R., OLIVEIRA, R.P. AND PENA, S.D.J. (2004) TRYPANOSOMA CRUZI: GENETIC STRUCTURE OF POPULATIONS AND RELEVANCE OF GENETIC VARIABILITY TO THE PATHOGENESIS OF CHAGAS DISEASE. MEM. INST. OSWALDO CRUZ, RIO DE JANEIRO 99, 1-12

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Chagas (Trypanosoma cruzi) IgG – ELISA

Chagas (Trypanosoma cruzi) IgG – ELISA

The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.

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