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GenWay/Caspase-8 Fluorometric Assay Kit - 25 assays/GWB-AXR034/25 assays
2026-08-23
Description:
Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by FLICE or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Summary: - Detection method- Flow cytometry (400 nm excitation filter and 505 nm emission filter) and fluorescence microscopy - Sample type- Cell and tissue lysates- Species reactivity- Mammalian - Kit size- Convenient sizes (25, 100, 200, 400 assays)- Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.Kit components: - Cell Lysis Buffer- 2X Reaction Buffer- IETD-AFC (1mM)- DTT (1 M)Features and Benefits: - Simple one-step procedure; takes 1-2 hours- Fast and convenient- Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. Shipping Conditions: Gel packAdditional Information:
| Caspase-8 Fluorometric Assay Kit - 25 assays |
| caspase8, caspase 8, Caspase-8 Fluorometric Assay Kit, Caspase-8, Caspase-8 Substrate, IETD, IETD-AFC, Caspase-8 assay, Caspase-8 Fluorometric Assay |
| Assay Kit |
| -20C |
| Research Use Only |
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