Agrisera/PSA2 | Photosystem I assembly factor 2/AS13 2654/
| application information | ||
| Recommended dilution | 1 : 1000 with standard ECL (WB) | |
| Expected | apparent MW | 20 kDa | 10 kDa (processed form) | |
| Confirmed reactivity | Arabidopsis thaliana | |
| Predicted reactivity | Brassica rapa | |
| Not reactive in | no confirmed exceptions from predicted reactivity are currently known | |
| Additional information | to be added when available | |
| Selected references | Fristedt et al. (2014). A Thylakoid Membrane Protein Harboring a DnaJ-type Zinc Finger Domain is Required for Photosystem I Accumulation in Plants. J Biol Chem. 2014 Sep 16. pii: jbc.M114.587758. |
application example
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Respective amounts of a leaf total cell extract from Arabidopsis thaliana loaded on total chlorophyll µg/µl were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds. Courtesty of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands
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