AKT Antibody Properties DescriptionAnti-AKT (SHEEP) Antibody - 600-601-401 HostSheep Target SpeciesHuman Known Cross Reactivityhuman Clonality Polyclonal Application ELISA : 1:5,000 - 1:20,000 IF Microscopy : user optimized Flow Cytometry : User Optimized Western Blot : 1:500 - 1:2,000 Immunohistochemistry: user optimized Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice FormatIgG LabelUnconjugated Concentration 0.9 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.1% (w/v) Sodium Azide Stabilizer None GeneName AKT1 Relevant Links UniProtKB - P31749NCBI - 62241011GeneID - 207

AKT Antibody Description

BackgroundAKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research. Synonyms sheep anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 ImmunogenAnti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein.  A residue of cysteine was added to facilitate coupling. Immunogen TypePeptideStorage Condition Store Anti-AKT at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note This Anti-AKT affinity purified antibody is suitable for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user for IF and IHC.  Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research. Purity/Specifity This antibody is directed against AKT and reacts with the AKT from human tissues.  Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.