Ambra1 Antibody Properties Description Anti-Ambra1 (RABBIT) Antibody - 600-401-Y08 Host Rabbit Target Species Human Known Cross Reactivity Human, mouse, rat Clonality Polyclonal Application ELISA : 1:10,000 IF Microscopy : 20 µg/mL Western Blot : 1 µg/mL Immunohistochemistry: 5 µg/mL Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1 mg/mL by UV absorbance at 280 nm Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Preservative 0.02% (w/v) Sodium Azide Stabilizer None GeneName AMBRA1 Relevant Links UniProtKB - Q9C0C7GeneID - 55626NCBI - Q9C0C7

Ambra1 Antibody Description

Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist. Synonyms Ambra1 Antibody, DCAF3, WDR94, KIAA1736, Activating molecule in BECN1-regulated autophagy protein 1 Immunogen Anti-Ambra1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human Ambra1. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-Ambra1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 143 kDa in Western Blots of specific cell lysates and tissues. Purity/Specifity Anti-Ambra1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Ambra1 from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.