MCLAB/1 Kb DNA Ladder/kDNA-050/1 Ea
Description: The 1 Kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 9 kb. The ladder may be radioactively labeled by one of the following methods: (i) Partial exonucleolytic degradation and resynthesis with T4 DNA polymerase (this method is preferred because higher specific activity is achieved with less 32P input); (ii) Labeling the ends with T4 polynucleotide kinase; (iii) Filling in the recessed ends with E. coli DNA polymerase I or the large fragment of DNA polymerase I. This ladder is premixed with Gel Loading Dye, Blue (6X) for direct gel electrophoresis. Application: Determination of the molecular weight of nucleic acids. Concentration and Usage: Conc. 0.1 μg/μl and 5 μl per lane Storage: Store at room temperature. Stability: Store at room temperature. Recommended Storage Condition -20°C. |
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