MCLAB/BigDye Terminator 5X Sequencing Buffer/SBUF-110/1 Ea
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| MSDS |
Description:The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.Applications:DNA sequencingFragment analysisRecommended Storage Condition: 4°CRecommended Protocol:Prepare sequencing reactions (10µl rxn) according to the following:- Add dye terminator mix 0.25-0.5µl- Add 5X Sequencing Buffer 2µl- Add template (100-200 ng/µl) 1 µl- Add primer (3 pmol/µl) 1 µl- Add H2O to 10 µlCycle the reaction according to the following protocol: Step 1: 96°C 3 minutesStep 2: 96°C 10 secondsStep 3: 50°C 5 secondsStep 4: 60°C 2 minutes and 30 seconds(25 to 30 cycles from step 2 to step 4)Step 5: 4°C hold Notes:Other cycle conditions may work well for individual users. These conditions work well for most of the samples processed in our lab using reduced volume and reduced terminator mix concentrations. |
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