MCLAB/Exonuclease I, E. coli/NI-200/20,000 units
Description:Exonuclease I cleaves single-stranded DNA in the 3' -> 5' direction, releasing 5'-mono/di-nucleotides and leaving double-stranded DNA molecules and the 5'-terminus intact. The enzyme is processed through digestion and is inhibited by the presence of a 3'-terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium(1-3).Specific Activity: 185,000 U/mgApplication:- Removal of residual ssDNA, including oligos, from reaction mixesSource:Purified from a strain of E. coli that expresses the recombinant Exonuclease I gene.Supplied in:10 mM Tris-HCl100 mM NaCl1 mM DTT0.5 mM EDTA50% GlycerolpH 7.5 @ 25°C 10x Exonuclease I Buffer670 mM Glycine-KOH67 mM MgCl2100 mM 2-mercaptoethanol(pH9.5@25°C) Unit Definition:One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.Recommended Storage Condition: -20°CReference:1. Lehman, I.R. and Nussbaum, A.L. (1964) J. Biol. Chem. 239, 2628.2. Kushner, S.R. et al. (1971) Proc. Natl. Acad. Sci. USA 68, 824.3. Kushner, S.R. et al. (1972) Proc. Natl. Acad. Sci. USA 69, 1366. |
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