MCLAB/T7 DNA Ligase/T7DL-300/45,000 Weiss units
Description:T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl termini in duplex DNA. The enzyme will join blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.Application:- Joining of Okazaki fragments during replication- Completing short-patch DNA synthesis occurring in DNA repair processSource:A recombinant E. coli strain carrying the T7 DNA Ligase gene.Supplied in:20 mM Tris-HCl300 mM NaCl1 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°C10x Rapid Ligation Buffer:660 mM Tris-HCl100 mM MgCl210 mM DTT10 mM ATP75% PEG 6000pH 7.6 @ 25°C Unit Definition:One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi]. One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit. Specific Activity: 45 Weiss U/µg Recommended Storage Condition:-20°CReference:Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089. |
本文链接： https://www.ebiomall.cn/b377-mclab/info-1556444815.html
