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MCLAB/BL21(DE3)/BS-196/1 Ea-蚂蚁淘商城
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MCLAB/BL21(DE3)/BS-196/1 Ea

  
  2026-07-31
  
High yields, high efficiency, and no heat shock necessary.

DescriptionHigh yields, high efficiency, and no heat shock necessary.

Application

  • Ideal for expressing proteins in E. coli.
  • BL21(DE3) cells carry the lambda DE3 lysogen.

Recommended torage conditionThis product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.Recommended reaction conditions

  • Mix DNA with competent cells
  • Let stand on bench for 5 minutes
  • Direct plate

GenotypeF– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])ReferenceMiroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:Chemically

Transformation Efficiency (cfu/μg):>1x108

Blue-Whiet Screening:No

Strain:B

Reduces Recombination:No

Cloning Methylated DNA:No

Improves Plasmid Quality:No

Preparing Unmethylated DNA:Not suitable

T1 Phage Resistant:No

RecA Deficient:No

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