MCLAB/2X HotSybr qPCR Kit/HSM400/1 Ea
For Research Use Only.
Description:This is a high performance real-time PCR reagent. It utilizes MCLAB's proprietary quantitative PCR technology.Advantages:2x HotSybr PCR Reaction Mix products cut the total reaction time down to half. |
![\"2hosybrpcrkits.jpg\"](\"https://www.ebiomall.com/images/mclab/2hosybrpcrkits.jpg\")
Figure: Amplification of human GAPDH gene target with 2X HotSybr Real-time PCR Kit. Amplification curves are shown for ten fold dilutions of 0.0002pM to 20pM of plasmid. Insets show the dissociation profile and the standard curve data.![\"mclab_pcr_image002.gif\"](\"https://www.ebiomall.com/images/mclab/mclab_pcr_image002.gif\")
- Normally the total reaction time is 4350 seconds: 95°C, 10 minutes => (95°C, 15 seconds => 60°C, 60 seconds) x 50- For MCLAB's 2x HotSybr PCR Reaction Mix, the total reaction time is reduced to 2350 seconds: 95°C, 10 minutes => (95°C, 5 seconds => 60°C, 30 seconds) x 50Application:Sybr green dye based quantitative PCR: including DNA quantification, 2-step RT PCR, SNP analysis, etc.Primer and probe design: Appropriate software, such as ABI Primer ExpressTM, should be used.Recommended Reaction Conditions:95°C, 10 minutes -> (95°C, 5 seconds. -> 60°C, 30 seconds.) for 50 cycles -> melting curve.Recommended Storage Conditions: -20°CNotes:To achieve accurate quantification, it is highly recommended to (1) do replicates; (2) reduce pipetting error; (3) primer concentration from 100nM to 300nM; (4) run melting curve following amplification cycles.Reference: Higuchi R, Dollinger G, Walsh PS, Griffith R; Bio/Technology 10: 413-417, 1992本文链接： https://www.ebiomall.cn/b377-mclab/info-1558689430.html
