MCLAB/T3 DNA Ligase/T3DL-100/7500 Weiss units
Description:T3 DNA Ligase joins blunt ends and cohesive ends as well as repairs single-stranded nicks in duplex DNA. In the absence of 20-30% PEG 6000, T3 DNA Ligase displays a very low efficiency for blunt-ended ligation(1). T3 DNA Ligase displays a higher efficiency for joining A/T overhangs than C/G matched ends(1). T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM(1).Application:Catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl terminus in duplex DNA.Source:A recombinant E. coli strain carrying the T3 DNA Ligase gene.Supplied in:20 mM Tris-HCl300 mM NaCl1 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°CSupplied with:2x Rapid Ligation Buffer2x Rapid Ligation Buffer:132 mM Tris-HCl20 mM MgCl22 mM DTT2 mM ATP15% PEG 8000pH 7.6 Unit Definition:One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi]. One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit. Specific Activity: 30,000 Weiss Unit/mg Recommended Storage Condition: -20°CReference:1. Cai, Liang, et al. (2004) J. Biochem. 135, 397-403 |
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