MCLAB/Uracil DNA Glycosylase (UDG)/UDG-200/20,000 U
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Description:Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.Application:- Control of carry-over contamination in PCR (1)- Glycosylase mediated single nucleotide polymorphism detection (GMPD) (2)- Site-directed mutagenesis (3)- As a probe for protein-DNA interaction studies (4)- SNP genotyping- Cloning of PCR products (5)- Generation of single strand overhangs of PCR products and cDNASource:A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.Specific Activity: 77,000 U/mgSupplied With:10X UDG Reaction BufferIncubate at 37°C10X UDG Reaction Buffer:200mM Tris-HCl10 mM DTT10 mM EDTApH 8.0 @ 25°CSupplied In:10 mM Tris-HCl50 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°CUnit Definition:1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.Recommended Storage Condition: -20°CReferences:1. M. C. Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 93, 125-128 (1990).2. P. Vanghan, T. V. McCarthy, A novel process for mutation detection using uracil DNA glycosylase. Nucleic Acids Res. 26, 810-815 (1998).3. T. A. Kunkel, Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA. 82, 488-492 (1985).4. P. R. Devchand, et al., Uracil-DNA glycosylase as a probe for protein-DNA interactions. Nucleic Acids Res. 21, 3437-3443 (1993).5. P. M. Booth et al., Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase. Gene. 146, 303-308 (1994). |
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