MCLAB/Poly (A) Polymerase/PAP-10/1,000 units
Description:Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3'-hydroxyl of RNA. The reaction requires Mg2+ and is template independent.Application:- Labeling of RNA with ATP or cordycepin- Poly(A) tailing of RNA for cloning or affinity purification- Enhances translation of RNA transferred into eukaryotic cellsSource:The gene encoding E. coli Poly(A) Polymerase expressed in E. coli.Supplied in:25 mM Tris-HCl500 mM NaCl1 mM MgCl20.1 mM DTT0.1 mM EDTA50% GlycerolpH 8.0 @ 25°CSupplied with:10x Poly(A) Polymerase Reaction Buffer10x Poly(A) Polymerase Reaction Buffer:500 mM Tris-HCl2.5 M NaCl100 mM MgCl2pH 7.9 @ 25°CUnit Definition:1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.Recommended Storage Condition: -20°C |
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