MCLAB/T4 DNA Polymerase/T4DP-300/3,000 units
Description:T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5' -> 3' direction. This enzyme exhibits a powerful 3' -> 5' exonuclease activity, while lacking any inherent 5' -> 3' exonuclease or strand displacement functions.Specific Activity: 5,555U/mgApplication:- 3'-overhang removal to form blunt ends- 5'-overhang fill-in to form blunt ends- Single strand deletion for sub-cloning- Second strand synthesis in site-directed mutagenesis- Probe labeling using replacement synthesisSource:Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.Supplied in:100 mM KPO41.0 mM DTT0.1 mM EDTA50% GlycerolpH 6.5 @ 25°CSupplied With: 10x Blue Buffer10x Blue Buffer:500 mM NaCl100 mM Tris-HCl100 mM MgCl210 mM DTTpH 7.9 @ 25°CUnit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-precipitable material in 30 minutes at 37°C.Recommended Storage Condition: -20ºCReference:1. Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.10-3.5.12. 2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd Ed.), 5.44-5.47.2. Dale, R., McClure, B. and Houchins, J. (1985) Plasmid, 13, 31-40.3. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382. San Diego: Academic Press.4. Panet, A., van de Sande, J.H., Loewen, P.C. and Khorana, H.G. (1973) Biochemistry, 12, 5045-5050. |
本文链接： https://www.ebiomall.cn/b377-mclab/info-1565590409.html
