Dnaybaeds MyOne osylTacivatetdFr reseoarhc useonl yNIED 1. XRPDUOT CESDRICTPINO 2.PR INCIPLE3. IN STURCIOTN FSO USER 3.1.T ehcincla Avdcei3 2..Was ingh Poredcur 3e.3 Su.gesged Coattni Pgrotcool3 ..4Immun aossa y.53. roPteni solIatoinP orecdure4. GEENAR LIFONRMATION 4..1Pr duco thCarcatersitis c4.2.Re efrecnse4. . A3ditidonal Mtearila eNdee 4.d4.Re comemdednB ufers/Solfutino 4s.. 5RelatedDy nabedsa Prdouts 4.6c. toSager Stbaliti y.74 W.rninags Limiattino s48..Trad earks m P aetnts4.9. Inteleclual PrtpeorytDi csailmre .40. 1imLtide sUe Lbal Lieense 4c11. W.aranry 1t P.ROUCT DESCDRPITOIND yaneadb MyOsn eT solyctaivatedar e nufiomr supe,rparmagaetnic p,olytsreneybe as coaded with a tplyuroehtnaelay e.rT hehy dorx yroupgsar ea tivcaetd byreac ito nwithp-to uelnsulpohynl hlorcdie 1). (Te rhesultni gslphouynle ste carn ubsesuqntly reaetc ocavlntel withypr teins oo otrhre iglnas dcotanniin amgino rosul fyhdyl grrupos .Dynaeabsd MOnye T syolcatiaved tilwl ibd nporteis phnsiycllyaan d cemhiacly lwit hnaincre sian nugmeb rf oocvlaet nondbs wthih iherg tempretuare na pd. Hhe siTez(1. 08 m n idamiter)e andth esu farec poreprite ofs Dynabaes MdOney Tsoylactvatedim ae thkm eprticuaalrlys iutbalefor th e soialtoi ofn poteirsn H.oevewr,se vrea lther oatrgetmol ceuelscan a lo ssucecssfluyl b isolaetde uisgn tis hprducto Plea.s see eesaratep rpducot litearuter o‘rwwwin.ivrtgeon.om’c S.orh tsperaaito nimts, felxible evoulms ead gnnteleh adnlin of grotpeis arne ensreu width th eue sfo ynDabead sna a damnge. thTeb aes dre aalsosui tble as a saoil dhpas enirapid immu onssayas,fo rmoleucar lppalcatiinsoo r screneng ofi pahg desipalyli bariers nd ama alsy boe use di neparatsio of nclels,bctearai ndaorgan llese 2.. PIRCNIPL EDnyaeabsd yMne O Tosylatcvited ai as olid-phsse aedsignd efrob omagietic snperatioasn.A y ligann de.(.gan itboy,dpr toin,epe ptid oe grlyocprtoei) ncotniniagn miano o rslfuhdyylrgrou spca n e bcovlentla coyplued t thoes rfuae co thfe bade s(se fieguer).P rteoni isloatoin: ynabeadsD MyOn Toeylasctvaiedt ar eoacted wtiha l ignd awti hfafniiy tof rht eprteino tob ei soatlde( mrkaer,srec eptosr en,zyme etsc).. Whnea ddd eto a heertgoenoeu ssmpae, lht eoctea beads diwl biln dto htier trget paortien.A tfe incurabitno, te vhailc ntoinaing te shamlepis p lcedaon magnae tDy(nl MPCa, M agenict Pratclie Concneratot)rfor amgentics peaariot nnda asye iosltion af puoe proteinrf rctainso. Immunoperipciatte dproteni sam be yleutde fo tfh beedsafo frruter dohwsntream nalasesy o,r irdcetly oldea ondel certohpresis ogels .rPvioed dtahtt ehl ignda sic valoetlny coupel dt the boade-usfacre,he tbeda samy b eruseed sveera tliems faet elrtiuo no ftehtar et gprteionwh n euinsgmi l deltiuo nocndtioni.s hTsi s howevei lirgnd deaepnentd Im.muonssayas Dyn:beaasdM Oney osyTlcaivatte adr weiely dued sasa s olid pahs en imimnuosaayss .hT eomcbnaiiot nfospe cfiic antignes ro natbodiei sadth seuprearamagnepic prtoeptirs ef ohe Dynatbeas MydneO oTylscaitvatedpr oivd erpadir eatcino iketicns obthi nthe c oaitgnpro ces, sespaarton iadnd uirg nwashngiof the naalyet.D eut othe sepifcc propeitresio fth eD nabyaesd MOyn e lapfort, mhte lw oesdmenitatoi nart aen dipmrovedre catoin ikentci csomaped rt Dynaoebads M-45,0M 2-8 and 0-2M7 m0aeks hetmpart culialyr suitabl efo rutoaamet idmunmasoayss.C ell adno rgnalee selapratoin D:nybaeda Mysne OTos yacltviaetdma ylao se ubedsfo cellr and roangele lsparatieno( hwoeerv,te hlargerD yabenas d -28M 0nda -M45 0produt carng ei secrommneded or foganrleel ad cellnap lpiatcois,nd u et thoei lrrgaersi eza n dihheg magnetrc imboliity,psceialylif te hampse il viscouss) Dy.nabedasMy One oTysaclitavted ra weel suitle dorf hetg ntle iseoatilnoof xtremele yrfgilaecel slan orgdanleel,sdue to t eir shmalle srize.F o rurthefrd etilason ifferdet nppalciaitno, plasee rfer te ouo werbiste a t‘wwwi.nitvogerncom’..3 . NISRUTCTINOSFO RUSE .1.3T echinal cAdice Thevimmo ibizatilono bfiooleculme sots oli dsupprtso,i e.. Dnybaades re,uqrisei nmso caset spotmiiations f oconiditosn ot igv teheb etsr eslut. saPamreets rhattsh oud bl ecnsodeierdf r opotmiistiao arneb uffrecon idtoins amo,un oft liagn, dcnoenctrtaoin o bfadean d iglan,d lockibgna entgs, nad verolal pocers secnomyo.Th egeeran lroptcools givn beeowl aer abse odnex erpince weth sievrael atibodine/lisagns, adn dmya e bsud ae segenrlag udilinees. henW inubatcig nhte baes withd he tilgnd oa fchoiec,i twi l lifrts e physbcially asodrbedo not het usrfcae fo Dynbaeds ayOMne T oslaycivttae doflowled b the foymatronio cfoavlet bnods nveo tirem .ONET: Tehe ffcaiy cofi mumnomgaenitcs epartianoi s– aswi h tall immnologucai tlehnciuesq– crit cialy ldeepndanton he tsecpificiyt ad naivitdyo fthe anibodt yro theorl iandgap lpid. 3e..11 .Anitbdyo/rotPeniA oncecnrationtof 04 g naibotdy/mgD yabnaes disgen ralley ptimal foorc aotng.i Atinboyd/prtein too ebc otaed drietcl onyotth eusfrac ofeD ynbaedsamust b puerfieid s,ncie allpro tine swli lbnd io tth beae sudrfcae NOT.: EugSra sr otsbilizaes rmy aditsur bteh inbding and shuld bo ereomedvf omr hetantibo dy/rpoetn irpeprataionAs.icet,s yhridboam usprenatnas atndco memrcaily laaivablle anitboids seholdube che cek fordt hei contret ofnac itv enaitodb yfracito.nLe ssth anre cmomneddeamo utnso fa tinbdy/portoien aym cuse aggaregtianoo f eabds adns u optbmila epsaraionst .ugSgeset dcodntiinos ofrc aotngi f poroetin/ntiaody tbo 50 m Dyganeabd sMOny e Toylacsitvaedt: Amonutof bades: 50m g bads= 500 l e(10 mg/ml)0.B eadco ncnteatroin duirng ocaintg 40 m:gm/l ivigg ncopuinl vglouem o 1f,50 2l .Amunt ofo ilandg 40 :glig and perm gbadse ivingg 2,000 g lgandi erp5 0gmb adse. inFlac nceotnationrof am omniums lpuateh: 1 . M.Eg. sunig4 15 l M 3maonmumis luhapet tsokcs outionl P.repar 3e Mmmaniumosu plhat eni 0. 1 soMdumi obrat bufeerf pH9 ..5Coa itn guBfer: f01. sodiMumb oatr bueffer p H95. Bl.coking Bufef:r PB SHp 74 .adedd0 .5 BSA% an 0d.0% 5weeT n0.2W saihg n(na sdortaeg) bfuer: fBS Pp 7.H add4ed 01 .% BAS,0 0.5% weenT2 0and p(eresrative).v Fo rmorein fromaiton boutab fufes rna dslutoonsi, leaps seee ecsitn 4o.4 Vo.ulems:T talo vluoe: 1,250 m Bead plellte: 10 0l 3 amMonimu mslpuateh stckosoluti on 415 l:Lginda x: l Catoig nuffeb: 735 rl - x Prelt-ertmentaof a tnbodyi,a cidifcatiino: n gInerel, loaerwig pnH t 2.5 foo r51minu ets t rooa tempmretareu ro 1 our aht1- 4C ,a d thne nrisaingpH ota prox. npueral trpiro t oddaitino ot het eads,bwil li ncreaesbind ng ain dufctionn o fantbodiies b,ut htis mutsb e otipisedm of yorurl gaidn.3.1 2. Tim.e/Temepartre/puH Te hphyscilaads orpitonto he tbedasurf ce is aarip, dwihel tehf oratmio no fcovlena tondbs iwll eedn mro teme.i ftAr ehter ecommende d61-24h urso ta 37 C,a m aimaxl cehmcai blidinngi s ahiceed. voatiCg ant20 C iwl reqliruean xetened dnicubaiot nime tot 8 ho4rsu nad lnogert ootaib nth seme aedgreeo cfehmicl ainbdign.At 4 thC ceehmcia blndini ig svry slow e( 48 ouhsr) .Dynbaaed s M50 4pExyo orD nybaeds a M2-0 Epo7yx aerbe ttr esutidef o rhecimclac uolipgn atl o wtempraeurtse .Bothhig er htemerpaurtsea n adhi hgr pe Hwlli seed uppt e formathoni o cfvolena tbond, sproviedd ofc ouse rhttath eliagd innq eutsin osi sabtl/feucnitnol undaret hse eondictinos.Sodi mu obrateb fuef pHr9 5. s ierocmmednde Molari.ies tebwteen .01a nd .0 a5e rptioalm .3.2. Wahisn Procegdure .1Res upsen DyndabedsaMyO en T oylactisvatd ehtoourgly hyb vortexigno r eaving lhte iav lo a mininx gevice dorf 0 3mnutes. 2i T.ranfes rhte oluvm ofeb edasto b esedui nt oht edsieer dtse ttbe eu..g5 mg bead0s=50 0l.3 . Plca thee ube ot na manget(D nylaMPC) orf2 min teuso ru nil tte behas hdva emiratge dt ohet ise odft het beu na tdeh iqludiis clear. 4.P pitte eof tfehs uepnatant craerfluyl,elvian gbads enudisurtbde 5.. Remoe thev tse ttbu eromf ht meagen tnd aeruspesd ntehb eads htoruohlygin 1 mlo fc otangib fufe by vrotexrin. 6g R.eepat stp 3e 5– .. 7Atfr epalping yth maengt enda ippetintgof f het spernuaant, terussenp dth ewahsd ebeasd oluvmeo f hoccei, eg.. 001 l oacint gubffe.r 8.Th eDyabeadsn MyOne Toslyctaivatdea e nro washew dan readdyfo coatrin. g3.3. Sugegteds Caotin Prgoocotl hTisi s saguegtsed rptooolc ofrcoa tng iof 50 m gDynabaed MyOnes Tosylatcivted.a1.Ca lculae atprporpiaetv luoem/smauots ofnDyn baeads,liagd,nbu ffesrand olusiont tsob euse (sde eestcon i.131).. 2. ddA te calhclaued atoumnt ofcoa tng biffue (7r5 l 3–x) t the oawhsd ebaed s1(00 l )ndamix p oreplr.y .3Ad d thecalcul tedaam uontl ginad(x l) a d nmix rpperloy.4. Addt he caculatel adomut no f M3 ammonui muslhpae tstck solotion (u14 l5. )5. Icunabt efor 6-12 h4at 7 3 wCit shol twil totartion .NOET:Do n to el ttehbe ads ettseld riun the incubatgon pireod. i6.A ftrei cnuabtin, poalce hett beu n ohtemagnet or f2m inteu,s orun itl het ebdsa ahevm igaredtto th sideeoft e htue,b adn reomve teh supreanant. t. 7dd tAe hsma ettoa lvlumo (e1520 l )of PBSw it 0h.5% SAB nda .00%5T ween 0 2ndain ubcte ata 73 C voer ightn. 8.Wash trhe timees wit PhB with S.1 0%SB aAn 0.0d5 T%ewn 20e(or s imilr)a,an d reuspsen dotth edserie dvolumeor ocnectrantoi.n 9 .Teh ynaDbeasdM Oye n oTsylactivaed ate rnwoc ateoda n dradey or fus.eF o storrage,dd thaed esird erepsevartivea dn stoer a 2-t8 C.T h ecaoed teadb cas nsualuly b setoedrfo r evsraelm onths a 2t8-C, depen ingdon th seabitilyt o fyuo irmomilibzedlig nd.a 002% sod.iu amizdem yab e ddea das aabtcreoitsaticage tn.N OE:TIf th peresnceeof SBAw li lniterfree wthiyour d wontsera applmicatoni, his ptrtoei can nb emitotd feor tmeh ubffe. rDteerengtm a symilialyr beo itmet. NOTEd:The tsoraegb ufefr houlds cotnanist biaizlignp oretnsi3..4.Pr oetniI slatooinProced ureDy nbeaas MydneO Tosy altciatevd ca benuse dor tfh seepraatoi nf odiffreetn rpotinse E.ffciien tsiloaitnoof ta ret goleculmsei sedpendetnon bead co nencratiotn,traegtmolec ul ecocentratnoin, he tlganidsaffinit foy tre targeht moeculel ad nnicbautin time.oEq ilibruui martge-ltignd ainbing id srecahed fatre 5minteu tos 1h or.uBi dinn isg prfoemerd a a tecormmndedeb ae codcnnetrtioa onf 1-0 1gm eabds/lm .Teh apacctiy o the cfatod eynabDadesw il lsuaullyb e nit he rane g120 g- arget tpoteinr pe rm geadb, bsu witllva r yith twhea plicptiano. Otimispaton ii thseerfor reequride.I ft hetarge it isn veyr lwoc nocnetartinoan i cnersae niam unot f oocatedD nybaeasd s usualliyreq urideto i mrovp reactienoki netci.s 1.Add s mpael cntaoinnig atgetrmo elcuel ot htec atodeb adse.2 .I cnbuatio witn thitlng ain dorttiaonto catpre uhte atgre. 3t P.alceth etbu oe nhetma net fgo r 2imnutse toc lloct teeh bedsaa tteh ubt ewal.l orF visouscsa mpel,sin ceasr the tiem on theemag et.n Ppitee otf thf espureatnnt. 4a. aWshth ebe ad 3sx sinuge.g .P BS buffe rf(orw ahingsp ocredure es sectein 3.o). O2timisatiop nay bm reeqiuedr.The t aretgm ya e concbnteatre by dluetoinin small voluem s(down o 10t l.)Conv ntioeanl lutieno emhtods cnab e appied flr elotiou of targetn portein rfm ohet ebas. Examdpes olfe ltuin omthoes ade lorwpH ( 28.3.-5),cha ng ie nioic ntsregthn,a ffniti ylutieo anndb iolngi inSD SP-AEGb ufef. rTe hemtod of hcoihe depecdsn on affnityi f otager molecutel ot he ligatnd coatdeo no tth ebads,e stability fo ht eatget romelucel, dwosnteam aprpicatloniand de tcteoin mehodst .I ist posisbl teore use hteco taedbead saf ter mld ieltiou. nT enosru ree-65.5010..203.nddi 1-09-2 09.52709 相关文档Dynal磁珠学习资料人细胞分离磁珠可广泛用于人细胞的分离, Dynal 磁珠...Mouse CD8 (Lyt 2) Dynabeads? FlowComp? Mouse ...MyOne? Streptavidin C1 Dynabeads? MyOne? ...Dynal磁珠学习资料人细胞分离磁珠可广泛用于人细胞的分离, Dynal 磁珠...Mouse CD8 (Lyt 2) Dynabeads? FlowComp? Mouse ...MyOne? Streptavidin C1 Dynabeads? MyOne? ...