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Enzolifesciences/Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)/BML-PW8810-0100/
2025-06-21
Description:
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated and monoubiquitinylated proteins regardless of linkage location but not free ubiquitin.Product Specification:
| Clone: | FK2 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Poly-ubiquitinylated lysozyme. |
| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC) |
| Species reactivity: | Species independent |
| Specificity: | Recognizes mono- and polyubiquitinylated conjugates. |
| Crossreactivity: | Does not cross-react with free ubiquitin. |
| Applications: | ELISA, IHC, IP, WB |
| Recommended Dilutions/Conditions: | Immunoprecipitation (care must be taken as MAb to Mono- and polyubiquitinylated conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilized)Western Blot (1:100-1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Purity Detail: | Purified. |
| Formulation: | Liquid. In PBS containing 0.1% sodium azide. |
| Handling: | Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
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