RhoA Activation Assay Kit 信号传导分子激活检测试剂盒-蚂蚁淘商城

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RhoA Activation Assay Kit 信号传导分子激活检测试剂盒

2024-04-30

货号：	80601
数量：	大量
英文名：	RhoA Activation Assay Kit
供应商：	NewEastbio
保质期：	2年
保存条件：	-20℃
规格：	20T

Product DescriptionSmall GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-familyof GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases theactivity of RhoA, and the hydrolysis of GTP to GDP renders it inactive.Currently the activation of RhoA proteins is assayed with the binding of GTP-bound RhoA to theRhoA-binding domain (RBD) of the RhoA effector protein, Rhotekin. This method is based on theobservation that the active, GTP-bound RhoA could bind to the RBD of rhotekin. However, thereproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP toGDP during the assay procedure, and the low binding affinity of RBD to RhoA-GTP.NewEast Biosciences RhoA Activation Assay Kit is based on the configuration-specific monoclonalantibody that specifically recognizes RhoA-GTP, but not RhoA-GDP. Given the high affinity ofmonoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time.This assay provides the reliable results with consistent reproducibility.This anti-RhoA-GTP monoclonal antibody can also be used to monitor the activation of RhoA in cellsand in tissues by immunohistochemistry.NewEast Biosciences RhoA Activation Assay Kit provides a simple and fast method to monitor theactivation of RhoA. Each kit provides sufficient quantities to perform 20 assays. Assay PrincipleNewEast Biosciences RhoA Activation Assay Kit bases on the configuration-specific anti-RhoA-GTPmonoclonal antibody to measure the active RhoA-GTP levels, either from cell extracts or from in vitroGTPγS loading RhoA activation assays. Briefly, anti-active RhoA mouse monoclonal antibody will beincubated with cell lysates containing RhoA-GTP. The bound active RhoA will then be pulled down byprotein A/G agarose. The precipitated active RhoA will be detected by immunoblot analysis usinganti-RhoA rabbit polyclonal antibody. 温馨提示：不可用于临床治疗。

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