长期批量供应 —— 采用重组技术，可实现快速生产 首次实验即可成功 —— 经过大量验证确认了特异性 符合伦理标准 —— 产品不含动物成分 This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%. Rat, Human预测可用于: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey Synthetic peptide:MDGTGLVVERDFSlinked to a carrier protein by a carboxyl terminal Cysteine residue linker. Sequence corresponds to amino acids 9-21 of Human Dopamine Receptor D1. Run BLAST withRun BLAST with 常规说明 The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As 存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. The Abpromise guarantee Abpromise™承诺保证使用ab40653于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 WB 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Dopamine Receptor D1 peptide (ab137968). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. 细胞定位 Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilutionLane 1 : Human brain tissue lysate - total protein (ab29466)Lane 2 : Rat Cortex Tissue LysateLysates/proteins at 10 µg per lane.SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size: 49 kDaAdditional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.Exposure time: 90 seconds 发表研究结果有使用 ab40653？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab40653 被引用在 10 文献中. Eom TY et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun 11:912 (2020).PubMed: 32060266 Williams MT et al. Whole brain proton irradiation in adult Sprague Dawley rats produces dose dependent and non-dependent cognitive, behavioral, and dopaminergic effects. Sci Rep 10:21584 (2020).PubMed: 33299021 Regan SL et al. Knockout of latrophilin-3 in Sprague-Dawley rats causes hyperactivity, hyper-reactivity, under-response to amphetamine, and disrupted dopamine markers. Neurobiol Dis 130:104494 (2019).PubMed: 31176715 Wang T et al. Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice. J Neuroinflammation 15:2 (2018). MousePubMed: 29301581 Zhao Y et al. Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala. BMC Biol 15:128 (2017).PubMed: 29282124 Cheng HW et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis 6:e1753 (2015). HumanPubMed: 25950483 Han M Kim J Effect of dietary iron loading on recognition memory in growing rats. PLoS One 10:e0120609 (2015).PubMed: 25746420 Rothmond DA et al. Developmental changes in human dopamine neurotransmission: cortical receptors and terminators. BMC Neurosci 13:18 (2012). HumanPubMed: 22336227 Burke AR et al. Adolescent social defeat increases adult amphetamine conditioned place preference and alters D2 dopamine receptor expression. Neuroscience 197:269-79 (2011).PubMed: 21933700 Schlenker EH Schultz HD Hypothyroidism attenuates SCH 23390-mediated depression of breathing and decreases D1 receptor expression in carotid bodies, PVN and striatum of hamsters. Brain Res 1401:40-51 (2011).PubMed: 21669406 Blocking step normal donkey serum/PBS as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 22 C Thank you for your patience. I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655). The immunogen peptide for ab40653 can be found with the catalog number ab137968 https://www.abcam.com/Dopamine-Receptor-D1-peptide-ab137968.html (or use the following: https://www.abcam.com/Dopamine-Receptor-D1-peptide-ab137968.html). The immunogen peptide for ab40655 can be found with the catalog number ab137997 https://www.abcam.com/Dopamine-Receptor-D3DRD3-peptide-ab137997.html (or use the following: https://www.abcam.com/Dopamine-Receptor-D3DRD3-peptide-ab137997.html). I hope the peptides are useful for you. Please do not hesitate to contact us again, should you need any additional information or should youhave any other questions. I wish you a good weekend! Read More Thank you for your enquiry.I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.Should you have any further questions, please do not hesitate to contact us. Read More Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. https://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn\'t be too concerned about the linker on the immunogen.  Most customers will choose an antibody based on the data we have on the datasheet.  These antibodies are more or less the same in terms of immunogen, testing information, purity, etc. We have specifically tested both in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.  We have 2 references for ab20066: https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab20066-references.html For ab40653, we do know of a rat-specific reference in WB. https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab40653-references.html Essentially, we would guarantee either to work in the way you are testing, and these antibodies are essentially very similar. I hope this information helps.  Please contact us with any other questions. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com