前激肽释放酶缺乏血浆 – 冷冻缺乏前激肽释放酶

前激肽释放酶缺乏血浆由正常柠檬酸盐人血浆制成，该血浆已通过使用针对 PK 的抗体进行选择性亲和免疫吸附来耗尽前激肽释放酶 (PK)。

仅使用最高的前激肽释放酶优质柠檬酸盐血浆用作起始材料，在许多情况下，母体血浆可用作对照材料。血浆产品通常通过添加 HEPES 至 20 mM 终浓度进行缓冲，并以 1.0 mL 至升的数量提供。我们的 PK 缺陷等离子体可用于进一步制造或仅限研究用途的应用。它尚未经过验证可用于凝血酶生成测定。

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规格储存和保质期储存

低于-60°C

类型

冷冻

有效期

请询问

可用的FormatsCat #FormatPK-DP

1.0 mL 小瓶至散装体积

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品前激肽释放酶缺乏血浆 - 冻干

PK-LDP – 冻干前激肽释放酶缺乏血浆 – 1.0 mL

纤溶酶原缺乏血浆 - 冻干缺陷血浆

纤溶酶原缺乏血浆由正常柠檬酸化人血浆制成，该血浆已通过使用针对 PG 的抗体进行选择性亲和免疫吸附来耗尽纤溶酶原 (PG)。

我们的冻干纤溶酶原缺陷血浆已被进一步制成冻干形式，以便于运输和储存。冻干血浆颗粒可使用 1.0 mL 试剂级水重新配制，并仅用于研究用途。该产品尚未经过验证可用于凝血酶生成测定。

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规格储存和保质期储存

2°C 至 8°C

有效期

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可用格式Cat #FormatPG-LDP

1.0 mL

仅供研究使用。文档门ts产品插页更多信息安全数据表更多信息相关产品纤溶酶原缺乏血浆 - 冷冻

PG-DP – 冷冻纤溶酶原缺乏血浆 – 1.0 mL 或散装

纤溶酶原缺乏血浆 – 冷冻缺陷血浆

纤溶酶原缺乏血浆由正常柠檬酸化人血浆制成，该血浆已通过使用针对 PG 的抗体进行选择性亲和免疫吸附来耗尽纤溶酶原 (PG)。

仅使用最高的血浆优质柠檬酸盐血浆用作起始材料，在许多情况下，母体血浆可用作对照材料。血浆产品通常通过添加 HEPES 至 20 mM 终浓度进行缓冲，并以 1.0 mL 至升的数量提供。我们的纤溶酶原缺乏血浆可用于进一步制造或仅供研究用途的应用。它尚未经过验证可用于凝血酶生成测定。

请求分析证书

规格存储和保质期存储

低于-60°C

类型

冻结n

有效期

请询问

可用的 FormatsCat #FormatPG-DP

1.0 mL 小瓶至散装体积

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品纤溶酶原缺乏血浆 - 冻干

PG-LDP – 冻干纤溶酶原缺乏血浆 – 1.0 mL

PAI-1 缺陷血浆 – 冷冻缺陷血浆

PAI-1 缺陷血浆是使用针对 PAI-1 的抗体，从去除纤溶酶原激活剂抑制剂-1 (PAI-1) 的正常人血浆中制成的。

仅使用最高质量的柠檬酸盐血浆作为起始材料，并且在许多情况下，母体血浆可用作对照材料。血浆产品通常通过添加 HEPES 至 20 mM 终浓度进行缓冲，并以 1.0 mL 至升的数量提供。我们的 PAI-1 缺陷血浆可用于进一步制造或仅供研究用途的应用。它尚未经过验证可用于凝血酶生成测定。

请求分析证书

规格储存和保质期储存

低于-60°C

类型

冷冻

到期

请查询

可用的FormatsCat #FormatPAI-DP

1.0 mL 小瓶至散装体积

仅供研究使用。文件产品表更多信息安全数据表更多信息请点击下载：Affinity chromatography columns and media Selection guide
Affinity chromatography separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand attached to a chromatographic matrix. The technique is well suited for a capture or intermediate step and can be used whenever a suitable ligand is available for the protein(s) of interest. Affinity chromatography offers high selectivity, hence high resolution, and usually high capacity. Affinity chromatography is frequently used as the first step (capture step) of a two-step purification protocol, followed by a second chromatographic step (polishing step) to remove remaining impurities.
The target protein(s) is/are specifically and reversibly bound by a complementary binding substance (ligand). The sample is applied under conditions that favor specific binding to the ligand. Unbound material is washed away, and bound target protein is recovered by changing conditions to those favoring elution. Elution is performed specifically, using a competitive ligand, or non-specifically, by changing the pH, ionic strength, or polarity. Samples are concentrated during binding, and the target protein is collected in purified and concentrated form.
Affinity chromatography is also used to remove specific contaminants. For example, Benzamidine Sepharose™ 4 Fast Flow can remove serine proteases.
Chromatography media selection
Parameters such as scale of purification and commercial availability of affinity matrices should be considered when selecting affinity media.
HiTrap™ affinity columns are ideal for method optimization or small scale purification of target proteins using wellestablished protocols.
HiScreen™ columns are prepacked with a range of BioProcess™ chromatography media and are designed for
method optimization and parameter screening.
Affinity media can be prepared by coupling a ligand to a selected matrix. HiTrap NHS-activated HP is designed specifically to facilitate this process and is supplied with a recommended coupling procedure for coupling primary amines.
For separations of glycoproteins and polysaccharides, media screening may be required to select the correct specificity.
Immunoglobulins
While protein A and protein G affinity media are similar in many respects, their specificities for IgG differ. Protein G affinity media are the better choice for general purpose capture of antibodies since they bind IgG from a broader range of eukaryotic species and bind more subclasses of IgG. Species-specific examples include stronger binding of polyclonal IgG from cow, sheep, and horse to protein G. Polyclonal rat IgG, human IgG3, and mouse IgG1 are bound by protein G but not by protein A. Generally, protein G has greater affinity for IgG and minimal binding of albumin, which results in cleaner preparations and greater yield.
Conversely, protein A may be the better choice for isolating certain subclasses of IgG or for removing cross-species IgG contaminants from horse or fetal calf serum, for example.
Purification of human and mouse IgM is possible by the use of the HiTrap IgM Purification HP 1 ml column. The thiophilic adsorption medium with 2-mercaptopyridine coupled to Sepharose HP is designed for one-step purification protocols resulting in 80% to 95% pure IgM.
Purification of IgY from egg yolk is easily performed using HiTrap IgY Purification HP 5 ml column. This specially-designed medium gives over 70% purity in one step.
Tagged proteins
Tagged recombinant proteins present many practical advantages, the single most important being simple, onestep, high-purity affinity purifications.
Purification of tagged proteins is typically based on specific interactions between the tags and ligands. Four commonly used tags are: polyhistidine (His), glutathione-S-transferase (GST), Strep-tag™ II, and Maltose Binding Protein (MBP). Other tags include; Protein A, calmodulin-binding peptide (CBP), and biotinylated peptide. Histidine-tagged proteins have a high selective affinity for Ni2+, Co2+, and a variety of other immobilized metal ions, while the GST tag binds to glutathione ligands coupled to Sepharose. Histidine-tags are small and therefore less disruptive to the proteins on which they are attached. GST tags are larger and their removal from target proteins is often necessary.
Strep-tag II is a small tag of only eight amino acids. The tag binds specifically to the Strep-Tactin™ ligand immobilized on a Sepharose base matrix to yield pure target proteins. MBP-tagged proteins have high selectivity towards carbohydrates such as dextrin.
GE Healthcare offers a wide range of products for purifying histidine-, GST-, Strep-tag II, and MBP-tagged proteins. For example, tagged protein purification media and prepacked columns allow rapid, one-step purification of unclarified as well as pretreated cell lysates and cell-free systems. These media and prepacked columns permit manual purification with a syringe, a centrifuge, or by gravity-flow as well as automated purification with ÄKTA™ systems.

原文链接：http://www.bioku.net/archives/3210