驴抗羊免疫球蛋白 – 二抗抗体

Affinity 的驴抗羊免疫球蛋白 – 二抗经过免疫亲和纯化并与辣根过氧化物酶缀合。在含有 50% 甘油 (v/v) 的缓冲稳定剂溶液中，APIgG-HRP 浓度为 1 mg/ml。通过分光光度法测定，该缀合物的 Rz 比（Reinheitszahl，A403/A280）≥ 0.25。总蛋白为 0.5 mg。

宿主动物：驴

免疫原：从绵羊血清中纯化的完整 IgG（重链和轻链）。

特异性：免疫电泳该抗体在缀合前产生单一沉淀素线，其迁移率与纯化的绵羊 IgG 获得的相同。

应用：该产品适合作为绵羊 IgG 过氧化物酶缀合抗体的来源。

< img src=\"https://affinitybiologicals.com/content/user_files/sites/2/2022/02/Secondary-Antibody-14-scaled.jpeg\" width=\"1920\" height=\"2560\" alt />规格离子存储和保质期存储

-10°C 至 -20°C

类型

冷冻

过期

请咨询

可用格式Cat #FormatDAS-APHRP

1 x 0.5 mg

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品羊抗兔免疫球蛋白 - 二抗

SAR-APHRP – 羊抗兔免疫球蛋白，亲和纯化的 HRP 缀合 IgG（0.5 毫克小瓶）

A1AT 多克隆抗体 – Affinity PurifiedAntibodies

Affinity 的 A1AT 多克隆抗体 – Affinity Purified 是我们 A1AT（Alpha 1 抗胰蛋白酶，也称为 Alpha 1 蛋白酶抑制剂或 α1PI）抗体家族中最高水平的。在抗原亲和纯化过程中，IgG 已消除任何非特异性免疫球蛋白部分，这增强了剩余免疫球蛋白对靶抗原的特异性。结果是纯度非常高的产品，其滴度明显高于完整或纯化的 IgG。我们的 A1AT 多克隆抗体 – 亲和纯化以含有 50% 甘油 (v/v) 的 HEPES 缓冲盐水溶液形式提供，适用于免疫印迹、细胞免疫染色和多种类型的免疫测定等应用，其中信噪比较高需要使用这种浓缩产品达到的比例。

宿主动物：绵羊

查看物种交叉反应图表

规格存储和保质期

-10°C 至 -20°C 之间

类型

冷冻

有效期

请查询

可用格式Cat #FormatGA​​1AT-AP

1 x 0.5 mg

仅供研究使用。文档产品插页更多信息安全数据表更多信息相关产品Alpha-1-抗胰蛋白酶多克隆抗体

GA1AT-IG – 山羊抗人 α-1-抗胰蛋白酶，纯化 IgG（10.0 毫克小瓶）

Alpha-1-抗胰蛋白酶多克隆抗体 - 亲和纯化 - 绵羊

SA1AT-AP – 绵羊抗人 Alpha-1-抗胰蛋白酶，亲和纯化 IgG（0.5 毫克小瓶）

Alpha-1-抗胰蛋白酶多克隆抗体 - 亲和纯化 HRP 缀合

SA1AT-APHRP – 羊抗人 Alpha-1-抗胰蛋白酶，亲和纯化 HRP 缀合 IgG（0.1 毫克小瓶）

Alpha-1-抗胰蛋白酶 A1AT 多克隆抗体

SA1AT-IG – 羊抗人 α- 1-抗胰蛋白酶，纯化 IgG（10.0 毫克小瓶）

Alpha-1-抗胰蛋白酶多克隆抗体抗体

Affinity 的 Alpha-1-抗胰蛋白酶多克隆抗体是我们 A1AT（也称为 Alpha 1 蛋白酶抑制剂或 α1PI）抗体家族的基础水平。 IgG 的纯度通常为 90%，以含有 50% 甘油 (v/v) 的 HEPES 缓冲盐水溶液形式提供。滴度基本上与起始抗血清相同，并且每个小瓶通常含有从一毫升抗血清中回收的 IgG 量。这种 Alpha-1-抗胰蛋白酶多克隆抗体通常用于免疫沉淀、免疫电泳、免疫耗竭和活性中和测定等应用。

宿主动物：山羊

查看物种交叉反应性图表

规格储存和保质期储存

-10°C 至 -20°C

类型

冷冻

有效期

请查询

可用格式Cat #FormatGA​​1AT-IG

1 x 10.0 mg

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品A1AT 多克隆抗体 - 亲和纯化

GA1AT-AP – 山羊抗人 Alpha-1-抗胰蛋白酶，亲和纯化 IgG（0.5 毫克小瓶）

Alpha-1-抗胰蛋白酶多克隆抗体 - 亲和纯化- 绵羊

SA1AT-AP – 绵羊抗人 Alpha-1-抗胰蛋白酶，亲和纯化 IgG（0.5 毫克小瓶）

Alpha-1-抗胰蛋白酶多克隆抗体 - 亲和纯化 HRP 缀合

SA1AT-APHRP –羊抗人 Alpha-1-抗胰蛋白酶，亲和纯化的 HRP 缀合 IgG（0.1 毫克小瓶）

Alpha-1-抗胰蛋白酶 A1AT 多克隆抗体

SA1AT-IG – 羊抗人 α-1-抗胰蛋白酶，纯化IgG（10.0 毫克小瓶）

Alpha-1-抗胰蛋白酶 A1AT 配对抗体套装

A1AT-EIA – Alpha-1-抗胰蛋白酶配对抗体套装 – 5 板套装

别名:Expand▼ 32 kD; bK286B10; D8Wsu38e; heat shock protein 32 kD; heat shock protein 32kD; Heat shock protein; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; Hmox1; HMOX1_HUMAN; HO 1; HO; HO-1; HO1; Hsp32; 应用: WB 1:500-1:2000, IHC 1:200, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. 反应性: Human, Mouse, Rat 来源: Rabbit 克隆性: Polyclonal 纯化: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). 特异性: HO-1 Antibody detects endogenous levels of total HO-1. 免疫原: A synthesized peptide derived from human HO-1, corresponding to a region within the internal amino acids. RRID: AB_2837878 引用格式: Affinity Biosciences Cat# AF5393, RRID:AB_2837878. 形式: Liquid 1mg/ml 保存条件: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Immunogen: A synthesized peptide derived from human HO-1, corresponding to a region within the internal amino acids. Uniprot: Visit The Human Protein Atlas Gene ID: Gene Name: HMOX1 Molecular Weight: Observed Mol.Wt.: 33 kD. Predicted Mol.Wt.: 33kDa(Calculated).. Subcellular Location: Microsome. Endoplasmic reticulum. Tissue Specificity: P09601 HMOX1_HUMAN:Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). Description: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Subcellular Location: Microsome. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). 1). Ding S et al. Sensitive and Selective Measurement of Hydroxyl Radicals at Subcellular Level with Tungsten Nanoelectrodes. Anal Chem 2020 Jan 13 (PubMed: 31927939) [IF=6.785] 2). Waltz PK et al. Heme Oxygenase-2 Localizes to Mitochondria and Regulates Hypoxic Responses in Hepatocytes. Oxid Med Cell Longev 2018 Apr 12;2018:2021645 (PubMed: 29849867) [IF=5.076] 3). Waltz PK et al. Heme Oxygenase-2 Localizes to Mitochondria and Regulates Hypoxic Responses in Hepatocytes. Oxid Med Cell Longev 2018 Apr 12;2018:2021645 (PubMed: 29849867) [IF=5.076] 4). Luo DD et al. Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. Food Chem Toxicol 2018 Nov 10 (PubMed: 30423402) [IF=4.679] 5). Dai JM;Guo WN;Tan YZ;Niu KW;Zhang JJ;Liu CL;Yang XM;Tao KS;Chen ZN;Dai JY; et al. Wogonin alleviates liver injury in sepsis through Nrf2‐mediated NF‐κB signalling suppression. J Cell Mol Med 2021 May 12. (PubMed: 33982381) [IF=4.486] 6). Shi Z et al. Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Front Pharmacol 2020 Apr 22;11:483 (PubMed: 32390839) [IF=4.225] 7). Zheng H et al. Echinacoside alleviates hypobaric hypoxia-induced memory impairment in C57 mice. Phytother Res 2019 Feb 15 (PubMed: 30768741) [IF=4.087] 8). Huang S et al. Nepeta angustifolia attenuates responses to vascular inflammation in high glucose-induced human umbilical vein endothelial cells through heme oxygenase-1 induction. J Ethnopharmacol 2019 Mar 1;231:187-196 (PubMed: 30419276) [IF=3.690] 9). Lin X;Yang F;Huang J;Jiang S;Tang Y;Li J; et al. Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. Life Sci 2020 Jun 3;256:117901. (PubMed: 32504759) [IF=3.647] 10). Lu W et al. ApoE deficiency promotes non-alcoholic fatty liver disease in mice via impeding AMPK/mTOR mediated autophagy. Life Sci 2020 Apr 15;252:117601 (PubMed: 32304762) [IF=3.647] 11). Mu G et al. Antioxidative effect of Lactobacillus plantarum Y44 on 2,2\'-azobis(2-methylpropionamidine) dihydrochloride (ABAP)-damaged Caco-2 cells. J Dairy Sci 2019 Aug;102(8):6863-6875 (PubMed: 31178173) [IF=3.333] 12). Yang WC;Cao HL;Wang YZ;Li TT;Hu HY;Wan Q;Li WZ; et al. Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury. Neural Regen Res 2021 Aug;16(8):1574-1581. (PubMed: 33433486) [IF=3.171] 13). Zhaohui Xue et al. iTRAQ based proteomic analysis of PM2.5 induced lung damage. RSC ADV 2019, 9, 11707-11717 14). Wang X;Li T;Ma B;Zhao Z;Ni L;Zhang R;Niu S;Liu C; et al. Melatonin attenuates restenosis after vascular injury in diabetic rats through activation of the Nrf2 signaling pathway. Biochem Biophys Res Commun 2021 Apr 9;548:127-133. (PubMed: 33640605) 15). Pei J;Cai S;Song S;Xu Y;Feng M;Luo G;Wang Y;Sun F;Shi H;Xu S; et al. Normobaric hyperoxia plays a protective role against renal ischemia-reperfusion injury by activating the Nrf2/HO-1 signaling pathway. Biochem Biophys Res Commun 2020 Oct 29;532(1):151-158. (PubMed: 32838965) 16). Li TT et al. Effects of a high concentration of hydrogen on neurological function after traumatic brain injury in diabetic rats. Brain Res 2020 Mar 1;1730:146651 (PubMed: 31926128) 17). Qiu H et al. DL-3-n-butylphthalide improves ventricular function, and prevents ventricular remodeling and arrhythmias in post-MI rats. Naunyn Schmiedebergs Arch Pharmacol 2018 Jun;391(6):627-637 (PubMed: 29602954) 18). Zhou J et al. Protective effect of Dioscorea zingiberensis ethanol extract on the disruption of blood-testes barrier in high-fat diet/streptozotocin-induced diabetic mice by upregulating ZO-1 and Nrf2. Andrologia 2020 Jan 20:e13508 (PubMed: 31957918) 19). Sun M;He N;Lv Z; et al. Polysaccharides extracted from Cassia seeds protect against high glucose-induced retinal endothelial cell injury. Int Ophthalmol 2021 Mar 17. (PubMed: 33733282) 20). et al. Effects of Sancai Lianmei Particle on autophagy and apoptosis in testes of diabetic mice via the Nrf2/HO-1 pathway. Int J Clin Exp Med 2019;12(6):6720-6732 21). et al. Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells. 22). et al. Antioxidant peptides from Mytilus Coruscus on H2O2-induced human umbilical vein endothelial cell stress. 23). Zhang Y;Chen Y;Li B;Ding P;Jin D;Hou S;Cai X;Sheng X; et al. The effect of monotropein on alleviating cisplatin-induced acute kidney injury by inhibiting oxidative damage, inflammation and apoptosis. Biomed Pharmacother 2020 Jun 20;129:110408. (PubMed: 32574971) 24). et al. Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1α through the cAMP-CREB Pathway. 25). et al. Agrimoniin Sensitizes Pancreatic Cancer to Apoptosis through ROS-Mediated Energy Metabolism Dysfunction. AF5393-BP (Blocking peptide available as AF5393-BP) Price/Size : ￥2000/1mg. Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg). Function : Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody. Format and storage : Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Precautions : This product is for research use only. Not for use in diagnostic or therapeutic procedures. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.