Biocolor 胶原检测试剂盒（Sircol Soluble Collagen Assay ）北京群晓科苑生物技术有限公司 浏览人数：2669 供应商：北京群晓科苑生物技术有限公司 诚信指数：5点 了解更多：进入公司展台 使用范围：仅限科研使用，不能应用于临床 蚂蚁淘声明：以上所展示的信息由企业自行提供，内容的真实性、准确性和合法性由发布企业负责。蚂蚁淘对此不承担任何保证责任。Biocolor 可溶性胶原检测试剂盒BiocolorSircol Soluble Collagen Assay采用与胶原特异性结合的Sircol染料，主要用于定量检测哺乳动物组织中抽提的酸溶性胶原和体外培养哺乳动物细胞释放到培养基中的胶原。溶于酸、胃蛋白等的可溶性胶原均可检测，具体可检测的胶原类型为I-V型，VI-XIV型胶原也可检测，但由于难以制备标准曲线，还无法定量。Sircol染料不能区分不同类型的胶原，但可与胶原的[Gly-X-Y]n螺旋结构特异性地结合。随着胶原变性为凝胶，该染料与胶原的结合力减弱。非脊椎动物与胶原由于变性温度较低、羟脯氨酸残基较少等原因，结合染料的能力较弱，需要制备种属特异性的纯化标准品，以绘制标准曲线。实验步骤 绘制标准曲线 待测样本的制备：取50ul样本容易调整体积到100ul进行反应。根据反应的结果对样本浓度进行调整，使其在标准曲线范围内； 胶原 染料复合物的获得：加入1ml Sircol染料，混匀离心后，既得胶原 染料复合物，弃上清，去掉未结合的染料 加入1ml 染料释放剂，将胶原 染料复合物中的染料释放出来 吸光度测定：用分光光度计540nm出定量测定。或使用colorimeter测定产品订购Product Code: S1000120 assaysProduct Code: S5000475 assays参考文献1Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:Applied Biomaterials.2Arizmendi, N., Puttagunta, L., Chung, K. L., Davidson, C., Rey-Parra, J., Chao, D. V., Thebaud, B., Lacy, P. andVliagoftis, H. (2014) Rac2 is involved in bleomycin-induced lung inflammation leading to pulmonary fibrosis. RespiratoryResearch 15, 71.3Aschner, Y., Khalifah, A. P., Briones, N., Yamashita, C., Dolgonos, L., Young, S. K., Campbell, M. N., Riches, D. W.,Redente, E. F., Janssen, W. J., et al. (2014) Protein Tyrosine Phosphatase Mediates Profibrotic Signaling in LungFibroblasts through TGF- Responsiveness. The American journal of pathology 184, 1489 1502.4Banon-Maneus, E., Rovira, J., Ramirez-Bajo, M. J., Moya-Rull, D., Hierro-Garcia, N., Takenaka, S., Diekmann, F.,Eickelberg, O., K nigshoff, M. and Campistol, J. M. (2014) Wnt Pathway Activation in Long Term Remnant Rat Model.BioMed Research International 2014.5Bellaye, P.-S., Burgy, O., Colas, J., Fabre, A., Marchal-Somme, J., Crestani, B., Kolb, M., Camus, P., Garrido, C. andBonniaud, P. (2014) Anti-fibrotic Role of B-crystallin Inhibition in Pleural and Subpleural Fibrosis. American journal ofrespiratory cell and molecular biology.6Bellaye, P.-S., Wettstein, G., Burgy, O., Besnard, V., Joannes, A., Colas, J., Causse, S., Marchal-Somme, J., Fabre, A.,Crestani, B., et al. (2014) The small heat-shock protein B-crystallin is essential for the nuclear localization of Smad4:impact on pulmonary fibrosis. The Journal of pathology 232, 458 472.7Beyer, C., Zenzmaier, C., Palumbo-Zerr, K., Mancuso, R., Distler, A., Dees, C., Zerr, P., Huang, J., Maier, C.,Pachowsky, M. L., et al. (2014) Stimulation of the soluble guanylate cyclase (sGC) inhibits fibrosis by blocking noncanonical TGF signalling. Annals of the rheumatic diseases annrheumdis 2013.8Biancheri, P., Di Sabatino, A., Ammoscato, F., Facciotti, F., Caprioli, F., Curciarello, R., Hoque, S. S., Ghanbari, A.,Joe-Njoku, I., Giuffrida, P., et al. (2014) Absence of a role for interleukin-13 in inflammatory bowel disease. Europeanjournal of immunology 44, 370 385.9Birukawa, N. K., Murase, K., Sato, Y., Kosaka, A., Yoneda, A., Nishita, H., Fujita, R., Nishimura, M., Ninomiya, T.,Kajiwara, K., et al. (2014) Activated hepatic stellate cells are dependent on self collagen, cleaved by membrane type1-matrix metalloproteinase for their growth. Journal of Biological Chemistry jbc M113.10Bode, C., Kinjo, T., Alvord, W. G. and Klinman, D. M. (2014) Suppressive oligodeoxynucleotides reduce lung cancersusceptibility in mice with silicosis. Carcinogenesis bgu005.11Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cellproliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.12Chen, T., Nie, H., Gao, X., Yang, J., Pu, J., Chen, Z., Cui, X., Wang, Y., Wang, H. and Jia, G. (2014) Epithelial mesenchymal transition involved in pulmonary fibrosis induced by multi-walled carbon nanotubes via TGF-beta/Smadsignaling pathway. Toxicology letters 226, 150 162.13Chen, Y.-S., Lee, S.-M., Lin, Y.-J., Chiang, S.-H. and Lin, C.-C. (2014) Effects of Danshensu and Salvianolic Acid B fromSalvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production. Molecules 19, 2029 2041.14Chen, Y.-P., Chen, W.-C., Wang, K.-C. and Chen, C.-H. (2014) Effectiveness of synovial fluid mesenchymal stem cellsembedded in alginate beads for treatment of steroid-induced avascular necrosis of the femoral head. Journal ofOrthopaedic Science 1 10.15Choi, E. M. (2014) Sunflower seed extract enhances the differentiation of osteoblastic MC3T3-E1 cells. Food andAgricultural Immunology 25, 9 19.16Chung, C.-C., Hsu, R.-C., Kao, Y.-H., Liou, J.-P., Lu, Y.-Y. and Chen, Y.-J. (2014) Androgen Attenuates CardiacFibroblasts Activations through Modulations of Transforming growth factor- and Angiotensin II Signaling. InternationalJournal of Cardiology.17Conte, E., Iemmolo, M., Fagone, E., Gili, E., Fruciano, M., Genovese, T., Esposito, E., Cuzzocrea, S. and Vancheri, C.(2014) Thymosin 4 reduces IL-17-producing cells and IL-17 expression, and protects lungs from damage in bleomycintreated mice. Immunobiology 219, 425 431.18Dakin, S. G., Smith, R. K. W., Heinega ard, D., nnerfjord, P., Khabut, A. and Dudhia, J. (2014) Proteomic Analysis ofTendon Extracellular Matrix Reveals Disease Stage-specific Fragmentation and Differential Cleavage of COMP (CartilageOligomeric Matrix Protein). Journal of Biological Chemistry 289, 4919 4927.19Daryadel, A., Bogdanova, A., Gassmann, M., Mueller, X., Z nd, G., Seifert, B., Lehalle, C., Frossard, N. and Tavakoli,R. (2014) Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats byblocking the c-Raf/ERK1/2 signaling pathway. Journal of Cardiothoracic Surgery 9, 81.20Den Hartog, G. J., Qi, S., van Tilburg, J. H., Koek, G. H. and Bast, A. (2014) Superoxide anion radicals activate hepaticstellate cells after entry through chloride channels: A new target in liver fibrosis. European journal of pharmacology 蚂蚁淘声明：以上所展示的信息由企业自行提供，内容的真实性、准确性和合法性由发布企业负责。蚂蚁淘对此不承担任何保证责任。