返回突出显示的引文用于单分子散斑 (SiMS) 显微术的红外肌动蛋白探针相关页面CF® 染料跨越可见和近红外光谱的下一代荧光染料CF® 染料 SE/TFP EsterSHARE 文章与同事分享这篇文章。复制链接发送电子邮件单分子显微镜成像是研究细胞过程的有力工具。然而，有一些技术挑战需要克服，例如细胞自发荧光背景和荧光团太暗或不耐光。在他们最近发表在《传感器》杂志上的文章中，作者 Yamashiro 和 Watanabe 通过开发一种标有近红外染料 CF™680R 的新型肌动蛋白探针来应对这些挑战，他们将这种探针用于电穿孔介导的单分子散斑 (eSiMS) 显微镜。由于细胞在红外范围内往往表现出较少的自发荧光，因此选择 CF™680R 而不是 DyLight® 549 是因为它在近红外区域的激发和发射光谱离子。他们发现 CF™680R-肌动蛋白探针比他们测试的其他染料-肌动蛋白结合物更亮、更耐光。它还能够与绿色和红色荧光蛋白一起用于三色 SiMS。 CF™680R-肌动蛋白探针显示在板状伪足中随着肌动蛋白逆行流动而移动，表明该肌动蛋白探针的行为符合预期。此外，CF™680R-肌动蛋白探针使作者首次能够测量粘附连接中肌动蛋白的周转率。要阅读原文，请单击此处。Yamashiro 和 Watanabe。用于基于深细胞电穿孔的单分子散斑 (eSiMS) 显微镜的红外肌动蛋白探针。传感器。 17(7), 1545 (2017)。 Doi：10.3390/s17071545 通过 TagRFP-T-肌球蛋白调节轻链（MRLC，左）CF680R-肌动蛋白 SiMS（中）和合并图像（右）可视化的肌动球蛋白束的代表性图像。粉色箭头表示相关肌动球蛋白束中的 CF680R-肌动蛋白 SiMS与 AJ。酒吧 = 5 微米。来自 Yamashiro 和 Watanabe，传感器，2017 年。要了解更多关于明亮和耐光 CF™ 染料的信息，包括我们无与伦比的近红外染料选择，请单击此处。DyLight 是 Thermo Fisher Scientific 的注册商标。返回重点引用用于 POC 诊断的袖珍型闭环 PCR 系统相关页面PCR 和 DNA 扩增用于 qPCR 的荧光染料和预混液，采用 Biotium 的 EvaGreen® 染料EvaGreen® 染料，20X 水 Forget-Me-Not™ qPCR 预混液高性能、低成本EvaGreen® 和探针 qPCR 的预混液分享文章与同事分享这篇文章。复制链接发送电子邮件设计准确但易于使用、便携且具有成本效益的床旁 (POC) 分子诊断系统具有挑战性。在 Biosensors and Bioelectronics 最近的一篇文章中，Shu 和同事描述了他们的电池供电、袖珍型、闭环对流 PCR 装置，它集成了样品制备和产品检测。首先，将细菌样品装入毛细管中。细菌与万古霉素修饰的磁珠结合，然后被磁阵列捕获。细菌 DNA 通过光热解和振动释放。 DNA进入反应回路，毛细管连接到具有热梯度的金属三角形支架（一端为 95 °C，另一端为 60 °C）。无需主动加热和冷却系统即可进行对流 PCR，在此期间，无线荧光成像模块会监测 PCR 产物的荧光。作者使用 EvaGreen® 染料实时检测 PCR 扩增。作者报告说，该设备的运行时间不到 25 分钟，动态范围为 106 至 101 DNA 拷贝/μL，显示了概念验证准确、便携且负担得起的 POC 诊断。要阅读原始文章，请单击此处。B. Shu，C. Zhang，D. Xing。用于即时诊断的样本到答案的实时对流聚合酶链反应系统。生物传感器和生物电子学，2017 年。DOI：http://dx.doi.org/10.1016/j.bios.2017.06.014EvaGreen® 染料通过一种新颖的\"按需释放”机制与 dsDNA 结合。EvaGreen® 染料是唯一的qPCR 染料可同时用于实时时间 PCR 和高分辨率熔解曲线分析。它还在等温 DNA 扩增、液滴数字 PCR 等方面有应用。此外，EvaGreen® 染料无毒、无致突变性，对水生生物无害。要了解有关 EvaGreen® 的更多信息，请单击此处。GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed™ and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed™ without changing your existing imaging system.

GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.

For new users, Biotium recommends GelRed™ 10,000X solution in water, our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRed™ 10,000X solution in DMSO for established users of GelRed™ in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRed™ 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRed™ 3X in water that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10 mL.

FeaturesSafer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxicEasy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trashUltra-sensitive: Much more sensitive than EtBr and SYBR SafeExtremely stable: Available in water, stable at room temperature for long-term storage and microwavableSimple to use: Very simple procedures for either precast and post gel stainingPerfectly compatible with a standard UV transilluminator : GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)Perfectly compatible with downstream applications : Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.The Most Sensitive and Stable Precast Gel StainFigure 1.GelRed™ is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films. The Most Sensitive and Stable Post Gel Stain

Figure 2. GelRed™ displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRed™and SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.

Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer

Please also see our EvaGreen™, a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart \"release-on-demand” DNA-binding technology, EvaGreen™ has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRed™, EvaGreen™ has remarkable stability.

Note: *GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.

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Catalogue # 41002            0.5ml/unitCatalogue # 41002-1        10ml/unit

For more information on Biotium GelRed, please visit www.gelredaustralia.com.au