26131-01-Pinkerton立克次氏体染色法_燃料(EMS和Biotium产品)-上海桑戈生物科技有限公司
pinkerton立克次氏体染色法(pinkerton smethodforrickettsia)
染色结果:
立克次氏体-亮红(brightred)
细胞核-蓝(blue)
试剂盒内容物:
保存温度
货号
品名
规格
rt
26131-01
methylenebluesolution,1%aqueoussol
500ml
rt
26131-02
basicfuchsinsolution,0.25%,aqueoussol
500ml
rt
26131-03
citricacid,0.5%aqueoussol
500ml
rt
26131-04
gram siodinesolutionor
500ml
rt
26131-4a
lugol siodine
500ml
rt
26131-05
sodiumthiosulfate,5%aqueoussol
500ml
固定:用zenker s(#64123-05)固定,或者10%中性formalin缓冲液固定
切片:paraffinat6 msections
染色步骤:
1.deparaffinizeandhydratetodistilledwater.
2.ifzenkerfixed,remove zenker scrystals byplacingingram siodine(#26131-04)orlugol siodine(#26131-4a)for15minutes.rinseinwaterandclearinsodiumthiosulfate,5%(#26131-05)forthreeminutes.washinwaterfor10minutesorlonger.
3.stainovernightinmethylenebluesolution(#26131-01).rinsein95%alcoholfor5secondstopreventlossofbluecolor.rinsequicklyindistilledwaterfor2 3seconds.
4.stainfor30minutesinbasicfuchsin,0.25%(#26131-02).
5.decolorizerapidlyincitricacid,0.5%(#26131-03)for1 2seconds,nevermorethan3seconds.
6.differentiatefurtherinabsolutealcoholuntilnucleistandoutblueandrickettsiaclumpsred.
7.clearwith2changesofxylene(#23400)
8.mountwithpermount(#17986-01)orshur/mount(#17990-01)
id=\"zhantai_logo\" src=\"http://img44.chem17.com/1/20120809/634801206362173750.png\" alt=\"上海桑戈生物科技有限公司\" onload=\"\">pinkerton立克次氏体染色法(pinkerton smethodforrickettsia)染色结果:
立克次氏体-亮红(brightred)
细胞核-蓝(blue)
试剂盒内容物:
保存温度
货号
品名
规格
rt
26131-01
methylenebluesolution,1%aqueoussol
500ml
rt
26131-02
basicfuchsinsolution,0.25%,aqueoussol
500ml
rt
26131-03
citricacid,0.5%aqueoussol
500ml
rt
26131-04
gram siodinesolutionor
500ml
rt
26131-4a
lugol siodine
500ml
rt
26131-05
sodiumthiosulfate,5%aqueoussol
500ml
固定:用zenker s(#64123-05)固定,或者10%中性formalin缓冲液固定
切片:paraffinat6 msections
染色步骤:
1.deparaffinizeandhydratetodistilledwater.
2.ifzenkerfixed,remove zenker scrystals byplacingingram siodine(#26131-04)orlugol siodine(#26131-4a)for15minutes.rinseinwaterandclearinsodiumthiosulfate,5%(#26131-05)forthreeminutes.washinwaterfor10minutesorlonger.
3.stainovernightinmethylenebluesolution(#26131-01).rinsein95%alcoholfor5secondstopreventlossofbluecolor.rinsequicklyindistilledwaterfor2 3seconds.
4.stainfor30minutesinbasicfuchsin,0.25%(#26131-02).
5.decolorizerapidlyincitricacid,0.5%(#26131-03)for1 2seconds,nevermorethan3seconds.
6.differentiatefurtherinabsolutealcoholuntilnucleistandoutblueandrickettsiaclumpsred.
7.clearwith2changesofxylene(#23400)
8.mountwithpermount(#17986-01)orshur/mount(#17990-01)
>本文链接: https://www.ebiomall.cn/b78-biotium/info-1502434486.html

