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CF™405S 染料-Biotium|biotium荧光试剂|GelRed|CF染料|biotium-Biotium 中国区总代理-上海开放生物科技有限公司
2025-11-21
CF 405S is a blue fluorescent dye with an absorption peak wavelength that nearly coincides with the 405 nm blue diode laser line (Figure 1). In addition, the emission peak wavelength of the dye well centers within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF 405S dye results in much brighter signal than that using Alexa Fluor 405 (Figure 2). A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and -bungarotoxin are available for CF dyes. Much brighter than Alexa Fluor 405 due to better compatibility with both excitation and emission windows on common instruments Highly water-soluble pH-insensitive ![\"Biotium中国区总代理\"](\"https://biotium.com/wp-content/uploads/2013/11/CF405S-Spectra16-300x212.gif\")
Figure 1. Absorption and emission spectra of CF405S goat anti-mouse conjugate in PBS. ![\"Biotium中国区总代理\"](\"https://biotium.com/wp-content/uploads/2013/12/CF405S-flow-plot-300x240.gif\")
Figure 2. Intracellular staining of Jurkat cells was performed with mouse anti-CD3 or isotype control followed by goat anti-mouse conjugated to Alexa Fluor 405 (AF405) or CF405S with varying degrees of labeling (DOL, or dye molecules per antibody molecule). Fluorescence was analysed on a BD LSRII flow cytometer with 405 nm excitation and 450/50 nm emission filter. Bars represent the fluorescence of the geometric means of the cell populations. ![\"Biotium中国区总代理\"](\"https://biotium.com/wp-content/uploads/2013/12/CF405S-GAM-CF488A-GAR-CF555-Phalloidin-icc-300x261.gif\")
Figure 3. HeLa cells stained with mouse anti-Golgin 97 and rabbit anti-COX IV followed by goat anti-mouse CF405S (Golgi, blue), goat anti-rabbit CF488A (mitochondria, green) and CF555 phalloidin (F-actin, red).
Figure 1. Absorption and emission spectra of CF405S goat anti-mouse conjugate in PBS. Figure 2. Intracellular staining of Jurkat cells was performed with mouse anti-CD3 or isotype control followed by goat anti-mouse conjugated to Alexa Fluor 405 (AF405) or CF405S with varying degrees of labeling (DOL, or dye molecules per antibody molecule). Fluorescence was analysed on a BD LSRII flow cytometer with 405 nm excitation and 450/50 nm emission filter. Bars represent the fluorescence of the geometric means of the cell populations. Figure 3. HeLa cells stained with mouse anti-Golgin 97 and rabbit anti-COX IV followed by goat anti-mouse CF405S (Golgi, blue), goat anti-rabbit CF488A (mitochondria, green) and CF555 phalloidin (F-actin, red). 生物试剂 版权所有 2009-2020 Powered by 上海开放生物 ©2008-2017 www.ope-tech.com
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Biotium 中国区总代理-上海开放生物科技有限公司，专利产品和技术：CF染料新一代荧光标记染料，EvaGreen高分辨率溶解曲线饱和染料，NucView双功能荧光凋亡检测Caspase3/7，Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等，AllGlo探针合成染料（替代Taqman技术），细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。
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