
The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin (e.g. Human Fibronectin Solution (C-43060)) and vitronectin (e.g. Vitronectin (C-69201)) coating.
Key features:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:
Tissue | Tested Cell Lines | Cell Lines Origin | Remarks |
---|---|---|---|
Brain | BV2 | immortalized murine primary microglial cells | Coat with Fibronectin:1 µg/cm2 |
Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | Coat with Fibronectin:1 µg/cm2 |
Colon | HT-29 | human colon adenocarcinoma | Coat with Vitronectin:0.5 µg/cm2 |
Connective tissue | HT1080 | human fibrosarcoma | Coat with Fibronectin:1 µg/cm2 |
Liver | HepG2 | hepatocellular carcinoma of the human liver | Coat with Vitronectin:0.5 µg/cm2 |
Lung | A-549 | human lung carcinoma | Coat with Vitronectin:0.5 µg/cm2 |
Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D culture in C-28070 is recommended |
Peripheral blood | MV-4-11 | Human acute myelogenous leukemia (suspension) | No coating required |
Bone marrow | KG-1 | Human acute myelogenous leukemia (suspension) | No coating required |
Kidney | ACHN | Human Renal Cell Carcinoma | Coat with Fibronectin:1 µg/cm2 |
Brain | A172 | Human Glioblastoma | Coat with Fibronectin:1 µg/cm2 |
Skeletal muscle | C2C12 | Mouse Myoblasts | Coat with Fibronectin:1 µg/cm2 |
Skin | B16-F10 | Mouse Melanoma | Coat with Fibronectin:1 µg/cm2 |
Abelson murine leukemia virus-induced tumor | RAW264.7 | Mouse Macrophage | Coat with Fibronectin:1 µg/cm2 |
Brain, neuroectodermal | Neuro-2a | Mouse Neuroblastoma | Coat with Fibronectin:1 µg/cm2 |
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希望有大神赐教!谢谢!
指导意见:
毕竟阴道上皮是鳞状上皮,而输尿管,膀胱,尿道的上皮是柱状上皮,形态上是不一样的.所以您要是对您的结果出怀疑态度,首先:重新留尿,做一个复检(留尿时注意防止阴道分泌物污染);或者让值班医生分析一下是那种类型的上皮(鳞状还是柱状).所以不要担心这个结果.一般情况下污染的比较多.
病情描述(发病时间、主要症状等):
体检血常规中血小板计数343.血小板比积0.39.
尿检中白细胞计数26.2.上皮细胞计数10.30
心电图下写窦性心律,p-r间期偏短
想得到怎样的帮助:
有没有什么大问题
如题,求助。做肝癌细胞侵袭、迁移实验,用哪种细胞株比较好?
2:红细胞计数(RBC)(参考值:3.5~5.5),(单位:10^12/L)
3:血红蛋白浓度(HB)(参考值:120~160),(单位:g/L)
4:红细胞压积(HCT)(参考值:40~48),(单位:%)
5:平均红细胞体积(MCV)(参考值:80~97),(单位:fL)
6:平均红细胞血红蛋白含量(MCH)(参考值:26.5~33.5),(单位:pg)
7:平均红细胞血红蛋白浓度(MCHC)(参考值:300~360),(单位:g/L)
8:血小板计数(PLT)(参考值:100~300),(单位:10^9/L)
9:淋巴细胞比值(LY%)(参考值:17~48),(单位:%)
10:单核细胞比例(MONO%)(参考值:4-10),(单位:%)
11:中性粒细胞比例(NEUT%)(参考值:43~76),(单位:%)
12:淋巴细胞计数(LY)(参考值:0.8~4.0),(单位: 10^9/L)
13:单核细胞计数(MONO)(参考值:0.3~0.8),(单位:10^9/L)
14:中性粒细胞计数(NEUT)(参考值:1.2~6.8),(单位:10^9/L)
15:红细胞分布宽度(参考值:11~14.5),(单位:%)
16:血小板体积分布宽度(PDW)(参考值:9~18),(单位:%)
17:平均血小板体积(MPV)(参考值:7.4~12.5),(单位:fL)
18:大血小板比例(P-LCR)(参考值:10~50),(单位:%)

