Human TLR9 recognizes specific unmethylated CpG DNA motifs prevalent in microbial but not vertebrate genomic DNA, leading to innate and acquired immune responses. Stimulation of TLR9 triggers a signaling cascade leading to the activation of the transcription factor NF-κB, which controls the expression of an array of inflammatory cytokine genes.
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如题,求助。做肝癌细胞侵袭、迁移实验,用哪种细胞株比较好?
病情描述(发病时间、主要症状等):
体检血常规中血小板计数343.血小板比积0.39.
尿检中白细胞计数26.2.上皮细胞计数10.30
心电图下写窦性心律,p-r间期偏短
想得到怎样的帮助:
有没有什么大问题
你的第一部分,白细胞和中性粒细胞偏低,说明免疫力低,最近要多保养,不受凉,近期容易生病。不过数据也不是太低的,也不用过于担心,多吃多睡,过一段时间复查,可能会好转为正常。另外消炎药,止痛,退热药尽量不吃,这些药会加重白细胞下降的。
第二部分,最主要的红细胞和血红蛋白是正常的,总体看还是正常的。但是下面数据有点低,而且只低一点,问题不大,多吃点米饭,红枣,肉等,会好转的。
第三部分,血小板部分是正常的。
你这种检查单,最主要的是免疫力低下,注意多吃多睡,别受凉,少烦神少劳累,引起白细胞低的药物尽量不吃。
G418浓度之前师兄已经摸索出来,这次筛选直接用师兄浓度。
1、6孔板转染后多长时间开始进行G418筛选?
2、G418筛选是在6孔板中继续筛选还是转到24/96孔?大皿?
3、G418浓度什么时候减量
4、?筛选过程中荧光观察一小部分克隆不错,如何挑选出来?
5、整个筛选过程大约需要多长时间?
lipo3000筛选
谢谢大家!
目前想建立家系血标本的淋巴细胞的永生化细胞株,请问有哪些成熟的方法及protol,费用贵么?
希望有大神赐教!谢谢!

