Introduction The prevalence of diabetes and obesity has been on the rise for several decades and research has demonstrated that other parts of the body, including the cardiovascular system and kidneys, can be affected by diabetes. […]

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Catalog #80-CPTHU-E01.1SpeciesHumanRegulatory StatusFDA Class I Device.For In Vitro Diagnostic Use.Product DistributionAvailable WorldwideRange20 - 3000 pmol/LSensitivity2.95 pmol/LSizes96 WellsSample TypesPlasma, SerumIncubation Time2 hrSample Size25µLInstructions for UseClick here to viewSafety Data SheetClick here to view - Additional Information
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Measurement of C-peptide, a 31 amino acid peptide, is being used to further understand diabetes mellitus, hypoglycemia and insulinoma. C-peptide is a byproduct formed in the process involving a series of enzymatic cleavages of preproinsulin and proinsulin, with proinsulin being the immediate precursor of insulin and C-peptide. C-peptide and insulin are known to be released in equimolar amounts from beta cells of pancreas. Since the half-life of C-peptide is about 30 minutes compared to insulin which is only about 3 minutes, measuring C-peptide may be more attractive for indirectly estimating glucose stimulated insulin secretion, understanding beta cell function and/or identifying beta cell functional mass.
The C-Peptide ELISA is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.
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- CitationsTitleJournalYearcloseAcute hypoglycemia in healthy humans impairs insulin stimulated glucose uptake and glycogen synthase in skeletal muscle: A randomized clinical studyDiabetes2017JournalDiabetesYear2017AuthorsVoss, T.S.; Vendelbo, M.H.; Kampmann, U.; et al.Volume & Issue #Vol. 66, Iss. 7Read ArticlecloseDietary patterns and cardiometabolic and endocrine plasma biomarkers in US womenThe American Journal of Clinical Nutrition2017JournalThe American Journal of Clinical NutritionYear2017AuthorsAlessa, H.B.; Malik, V.S.; Yuan, C.; et al.Volume & Issue #Vol. 105, Iss. 2Read ArticlecloseHuman islets contain four distinct subtypes of beta cellsNature Communications2016JournalNature CommunicationsYear2016AuthorsDorrell, C.; Schug, J.; Canaday, P.S.; et al.Volume & Issue #Vol. 7Read ArticlecloseTransient suppression of TGF-beta receptor signaling facilitates human islet transplantationEndocrinology2016JournalEndocrinologyYear2016AuthorsXiao, X.; Fischbach, S.; Song, Z.; et al.Volume & Issue #Vol. 157, Iss. 4Read ArticlecloseBeta cell death is decreased in women with gestational diabetes mellitusDiabetology & Metabolic Syndrome2016JournalDiabetology & Metabolic SyndromeYear2016AuthorsKenna, L.A.; Olsen, J.A.; Spelios, M.G.; et al.Volume & Issue #Vol. 8, Iss. 1Read ArticlecloseAge-dependent impairment of glucose tolerance in the 3xTg-AD mouse model of Alzheimer"s diseaseFASEB Journal2015JournalFASEB JournalYear2015AuthorsVandal, M.; White, P.J.; Chevrier, G.; et al.Volume & Issue #Vol. 29, Iss. 10Read ArticlecloseDifferentiation of human skin-derived precursor cells into functional islet-like insulin-producing cell clustersIn Vitro Cellular & Developmental Biology - Animal2015JournalIn Vitro Cellular & Developmental Biology - AnimalYear2015AuthorsMehrabi, M.; Mansouri, K.; Hosseinkhani, S.; et al.Volume & Issue #Vol. 51, Iss. 6Read ArticlecloseIslet neogenesis associated protein (INGAP) induces the differentiation of an adult human pancreatic ductal cell line into insulin-expressing cells through stepwise activation of key transcription factors for embryonic beta cell developmentDifferentiation2015JournalDifferentiationYear2015AuthorsAssouline-Thomas, B.; Ellis, D.; Petropavloskaia, M.; et al.Volume & Issue #Vol. 90, Iss. 4Read ArticlecloseIsolated hyperglycaemia does not increase VLDL-triacylglycerol secretion in type 1 diabetic menDiabetologia2015JournalDiabetologiaYear2015AuthorsJohansen, R.F.; Søndergaard, E.; Sørensen, L.P.; et al.Volume & Issue #Vol. 58, Iss. 2Read ArticlecloseTreating diet-induced diabetes and obesity with human embryonic stem cell-derived pancreatic progenitor cells and antidiabetic drugsStem Cell Reports2015JournalStem Cell ReportsYear2015AuthorsBruin, J.E.; Saber, N.; Braun, N.; et al.Volume & Issue #Vol. 4, Iss. 4Read Article
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就好像撒一撮芝麻在培养基那么大的地方上,我们不能知道它会怎么落。
原代细胞产品专家齐氏生物提醒,原代细胞培养注意事项,供参考:
1、实验材料要新鲜,从活体分离材料后要低温保存,并尽快进行细胞分离实验。
2、无菌操作。操作时用的培养液,可加平时细胞培养液5倍含量的青链霉素。
3、用酶法分离细胞时,注意酶液的浓度和控制消化时间。
贴块法分离细胞时,注意动作要轻柔,不要伤到细胞组织,组织块边缘尽量平整有利于细胞游离。
4、培养液的选择。不同的细胞有对培养液中营养的要求不同,根据所分离细胞的特性选择。
不知道楼主明白没有,意思是说培养基上的癌细胞可能过密,导致营养缺乏,无法满足生长分裂的需要,所以进入G0期,暂时不生长分裂.
3,MHC基因位于第六号染色体上,MHC存在于人类和哺乳动物的细胞表面.根据MHC所编码的分子结构和功能不同而将其分为1,2,3类?.1,2类分子结构相似,主要功能是向T细胞呈递抗原,是被T细胞识别的重要靶分子.1类存在于除红细胞以外的机体所以细胞膜上,2类分子仅存在于成熟B细胞,一些T细胞和抗原呈递细胞膜上,3类分子不具备上述功能,属于血清蛋白.
现在楼主应该明白了,MHC是不参与抗原抗体特异性结合的,是专门用来呈递抗原的.
细胞株(cell strain) 是通过选择法或克隆形成法从原代培养细胞中获得具有特殊性质或标志物的细胞称为细胞株。一般认为,细胞株是用单细胞分离培养或通过筛选的方法,由单细胞增殖形成的细胞群。细胞株的特殊性质或标志必须在整个培养期间始终存在。
细胞系(cell line) 是原代细胞经首次传代成功后即为细胞系。泛指一般可能传代的细胞。其中能够连续传代的细胞叫做连续细胞系或无限细胞系,不能连续培养的称为有限细胞系。大多数二倍体细胞为有限细胞系。由原先存在于原代培养物中的细胞世系所组成。如果不能继续传代,或传代次数有限, 可称为有限细胞系(finite cell line), 如可以连续培养, 则称为连续细胞系(continuous cell line), 培养50代以上并无限培养下去。人类肿瘤细胞,在体外培养半年以上,生长稳定,并连续传代的即可称为连续性株或系。
不知道楼主明白没有,意思是说培养基上的癌细胞可能过密,导致营养缺乏,无法满足生长分裂的需要,所以进入G0期,暂时不生长分裂.
3,MHC基因位于第六号染色体上,MHC存在于人类和哺乳动物的细胞表面.根据MHC所编码的分子结构和功能不同而将其分为1,2,3类?.1,2类分子结构相似,主要功能是向T细胞呈递抗原,是被T细胞识别的重要靶分子.1类存在于除红细胞以外的机体所以细胞膜上,2类分子仅存在于成熟B细胞,一些T细胞和抗原呈递细胞膜上,3类分子不具备上述功能,属于血清蛋白.
现在楼主应该明白了,MHC是不参与抗原抗体特异性结合的,是专门用来呈递抗原的.
哪种要好,有什么要求吗?接种多少代的细胞?
1.培养基最好是培养原代细胞专门的培养基或说是配套的培养基; 2.关键还是生长因子等的浓度; 3.如果需要的话,需要明胶包被.
2.关键还是生长因子等的浓度;
3.如果需要的话,需要明胶包被.