Description:
p53 ResponsiveLuciferaseReporterRKOStableCellLineisderivedfromhumancoloncancer,andstablyexpressfireflyluciferasereportergeneunderthecontrolofthep53responseelement.Thiscelllineisanidealcellularmodelformonitoringtheactivationofp53Pathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
Thep53pathwayplaysacrucialroleineffectivetumorsuppressionbecauseofitscentralfunctionincellcycleregulation,DNArepair,cellularsenescence,andapoptosis,whichcanbeusedforpotentiallydevelopnewdrugtherapiesagainstcancer.UponactivationbyDNAdamage,oncogeneactivation,orhypoxia,p53bindstoitsDNArecognitionsiteonthepromoterregionsofthetargetgenesandregulatethegeneexpression. Signosishasestablishedp53luciferasereporterstablecellline,inwhichluciferaseactivityisspecificallyassociatedwiththeactivityofp53.Therefore,thecelllinecanbeusedasareportersystemformonitoringtheactivationofp53triggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.
Thecelllinewasestablishedbytransfectionofp53luciferasereportervectoralongwithG418expressionvectorfollowedbyG418selection.TheG418resistantclonesweresubsequentlyscreenedforetoposide-inducedluciferaseactivity.Theclonewiththehighestfoldinduction(35fold)wasselectedandexpandedtoproducethisstablecellline.
PrinciplebehindTFluciferasereporter. TFluciferasereporterstablecelllineutilizesartificialpromoterconstructstodriveluciferaseexpression. Thepromoterregioncanconsistsofmultiplerepeatsofacis-elementTFbindingsite,aDNAfragmentfromthepromoterregionofaknownTFdownstreamgene,oraDNAfragmentcontainingputative/knownTFbindingsites. ThereareseveralwaysthataTFcanbeactivated,suchasthroughextracellularstimuliorthroughintracellularsignalingpathways. Onceactivated,theTFtranslocatestothenucleusandofteninteractswithrelevantco-factorstodrivegeneexpression. Onceluciferaseisexpressed,itcangeneratelightinanenzymaticassayandtheamountoflightmeasuredispositivelycorrelatedwiththelevelofTFactivation. |
Data:
Analysisofp53PathwayReporterRKO StableCellLineinresponsetostimuli. Thecellswereseededona96-wellplateforovernightwithDMEMincluding10%FBS.Thecellsthenweretreatedwithorwithout2ug/mlQuinacrinerespectivelyinDMEMand0.1%FBSfor16hours.
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株，就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
建立稳定细胞株,一般是根据不同基因载体中所含有的抗性标志选用相应的药物对靶...专注整体实验·服务生命科学已开展了数千个实验外包项目
如果经费充足的话可以找公司包装病毒，如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
