The Wheaton Erlenmeyer Flask is made of Lexan Polycarbonate Resin that has outstanding mechanical, optical, electrical and thermal properties. This resin conforms to flame retardant standards and is scratch resistant. This resin also meets the requirements of United States Pharmacopeia (USP) XXII Class VI. The Flasks are manufactured in a class 10K clean room. The flasks are certified pyrogen-, DNase- and RNase-free and are packaged particulate free and wrapped in an easy tear, sterile bag
Wheaton offers both flat bottom and baffled Erlenmeyer Flasks. Both Erlenmeyer Shake Flasks are supplied standard with a patented vented DuoCap. The DuoCap, made of medical grade polypropylene, has a 0.22
| SKU | WPFPC0250S |
|---|---|
| Color | CLEAR |
| Capacity(mL) | 250 |
| Pyrogen Free? | Yes |
| DNAse, RNAse Free | Yes |
| Cap Color | Blue |
| Plastic Material | Polycarbonate |
| Cap Material | Polypropylene |
| Sterile | Yes |
| Cap/ Closure Size | 38-430 |
| Bottle Style | Erlenmeyer |
| Bottle Material | Plastic |
| Closure Style | Vented DuoCap |
Product Literature
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
推荐了解“主细胞库”“工作细胞库”这两个名词。
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
1.将外源基因插入慢病毒载体
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株

