- SynonymActivin A,INHBA
- SourceHuman Latent Activin A, His Tag (ACA-H424x) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ser 426 (Accession # AAH07858.1).Predicted N-terminus: His (pro) & Gly311 (mature)Request for sequence
- Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 13.0 kDa (mature) and 32.0 kDa (pro). As a result of glycosylation and Interchain disulfide bond, the protein migrates as 15 kDa (mature) and 43-48 kDa (pro) under reducing (R) condition, and 27 kDa (mature), 43-48 kDa (pro) and 60 kDa (pro & mature) under non-reducing (NR) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human Latent Activin A, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The bio-activity of Human Latent Activin A, His Tag (Cat. No. ACA-H424x) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically 47-66 ng/mL (Routinely tested).
- BackgroundActivin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion.Activins are nonglycosylated homodimers or heterodimers of various β subunits (βA, βB, βC, and βE in mammals), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a widely expressed homodimer of two βA chains. The βA subunit can also heterodimerize with a βB or βC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human βA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat βA.Activin is produced in the gonads, pituitary gland, placenta, and other organs. The bioactivity of Activin A is regulated by a variety of mechanisms. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization; Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation; Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and especially kidney. Activin A increased the expression level of type-I collagen suggesting that activin A acts as a potent activator of fibroblasts; Lack of activin during development results in neural developmental defects.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
推荐了解“主细胞库”“工作细胞库”这两个名词。
对于瞬时转染的检测:如果有报告基因,就通过报告基因的表达看转染成功与否;没有报告基因的话,就在24~96h内收获细胞通过RT-PCR和WB来鉴定。

