BMP-7
Bone morphogenetic proteins (BMPs) are a group of approximately 15 structurally related proteins belonging to the TGF-beta protein family. One member of this family is bone morphogenetic protein 7 (BMP-7). BMP-7 is a 431 amino acid protein that exists as a homodimer, linked by a single disulfide bond.
BMP-7 is expressed in a wide variety of tissues including bone marrow, spleen, thymus, heart, muscle, liver, kidney pancreas, prostate and lung, in embryonic tissues, as well as in the central nervous system (including spinal cord).
BMP-7 plays a major role in bone growth (osteogenesis) and regeneration by inducing collagen production in osteoblasts, the differentiation of stem cells and organogenesis during embryogenesis.
BMP-7 is also involved in the chemotaxis of monocytes and has neuroprotective and neurogenesis properties. BMP-7 may be used in the treatment of wound healing, tissue repair and bone fractures, for neuroprotection and neurogenesis in the CNS and in nerve or spinal injuries.
For a review of the role of BMP-7 please refer to Chen et al. (2004) Growth Factors 22(4):233-41.
ebiomall.com
>
>
>
>
>
>
>
>
>
然后你得确定,这个细胞株对于你得研究来说不可或缺,具有明确的代表性。
上面两个都没有问题的话,祝你顺利。
① 在构建载体时,目的基因直接整合到细胞染色体组上,最好不要通过先瞬转在筛选稳定细胞株的这种方法,因为转染效率没有保证
② 高表达载体的构建,哺乳动物表达量一直是它自身的缺点,最好根据高表达载体定向的驯化细胞,提高蛋白表达量
③ 细胞的选择,筛选稳定细胞株我们常用的细胞是CHO,中国仓鼠卵巢细胞,由于CHO具有诸多的优点因此适合用于筛选稳定细胞株,而HEK293细胞则常用于瞬时转染
④ 后期的筛选,双抗预防污染,筛选细胞的时候抗生素浓度一定要做预实验,而且转染的时候不能有抗生素,关于细胞转染 稳定细胞系构建的相关理论
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。

