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Description
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Description: Mouse monoclonal antibody to human Tumor Necrosis Factor alpha (TNF-α)
Purification: Protein G affinity purified
Product Type:Primary antibody, detection antibody in matched antibody pair
Target Protein: Human TNF-α
Immunogen: Purified recombinant human TNF-α
Fusion Myeloma: Sp2/0-Ag14
Specificity:This antibody recognizes human TNF-a in ELISA and Western blot.
Species Reactivity: Human, other species not tested
Host / Isotype: Mouse, IgG1 Kappa
Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Store at -20oC
Research Area: Cytokine, inflammation
Background:
Tumor Necrosis Factor-alpha (TNF-α) is a multifunctional pro-inflammatory cytokine, mainly secreted by activated macrophages. It is implicated with a variety of biological procedures including systemic inflammation, cell proliferation, apoptosis, lipid metabolism, and coagulation. The pleiotropic attribute of TNF-α regulation is associated with its ability to trigger multiple signalling pathways through its receptors, TNFR1 (p55) and TNFR2 (p75).
TNF-α not only contributes to the immune response to bacterial, fungal, viral and parasitic invasions, but also functions in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. TNF-α hyper-expression in response to some bacterial components such as LPS can cause life threatening septic shock. Recombinant TNF-α, in combination with chemotherapy, has been applied for treatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-α therapy has been used for treatment of rheumatoid arthritis.
Applications:
ELISA:When clone B1E4 is used as detection antibody and clone CH8820 (Cat. No.: MO-C40003B) is used as capture antibody in an ELISA assay that uses WHO reference material, the detection limit of the ELISA can reach 2.4pg/mL TNF-α.
Western Blotting
Figure: Western blot analysis of recombinant human TNF-α using anti-human TNF-α mAb B1E4.
References: If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human TNF-α, B1E4, Detector |
|---|---|
| Application | ELISA, WB |
| Size | 0.1 mg |
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最近因工作需要，欲购进一些生产用的细胞株和瞬转、稳转质粒，大家有什么推荐的品系和公司或网站，谢谢！！！
主要需求：
1.生产或生物制药用的，稳定细胞株如CHO-K1、GS和HEK293/HEK293T，以及与之对应的瞬转、稳转（重组整合）质粒（需详细图谱）。
2.原始或改造细胞株，以及对应质粒，改造细胞株请详述细节，原因，优缺点，安全性等。
3.希望大家积极推荐，实验用、生产用均可，原核、真核都行，网站或品系名称也行，只要大家觉得常用、稳定、安全、高产就行。
4.请有意向的同仁回帖或将详细资料发至我163邮箱：wangqiang23mars@163.com，万分感谢。
