TMB ONE (& prestained)
ELISA HRP Substrate
Cat. No. 4380 / 4430
TMB (3, 3", 5, 5"-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).
TMB ONE characteristics are:
- “The Gold Standard” used across the industry
- Incredibly stable and robust
- Aqueous based – no organic solvents (REACH Annex XVII compliant)
- Low lot-to-lot variation
For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes:100 mL, 500 mL, 1 L , bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C in the dark
- Stability:4 years
- Development time:5-30 minutes
- Stop solution:0.2 M H₂SO₄
Downloads
Download your LOT specific Certificate of Analysis (CoA)

ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
对于瞬时转染的检测:如果有报告基因,就通过报告基因的表达看转染成功与否;没有报告基因的话,就在24~96h内收获细胞通过RT-PCR和WB来鉴定。
然后你得确定,这个细胞株对于你得研究来说不可或缺,具有明确的代表性。
上面两个都没有问题的话,祝你顺利。
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株

