
TMB ONE (& prestained)
ELISA HRP Substrate
Cat. No. 4380 / 4430
TMB (3, 3", 5, 5"-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).
TMB ONE characteristics are:
- “The Gold Standard” used across the industry
- Incredibly stable and robust
- Aqueous based – no organic solvents (REACH Annex XVII compliant)
- Low lot-to-lot variation
For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes:100 mL, 500 mL, 1 L , bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C in the dark
- Stability:4 years
- Development time:5-30 minutes
- Stop solution:0.2 M H₂SO₄
Downloads
Download your LOT specific Certificate of Analysis (CoA)

ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
ebiomall.com






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稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
建立稳定细胞株,一般是根据不同基因载体中所含有的抗性标志选用相应的药物对靶...专注整体实验·服务生命科学已开展了数千个实验外包项目
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。

