Intended Use:
The AESQC quality controls are reagents obtained from pools of human sera available with different autoantibodies that allow a multi parametric control in autoimmune disease diagnosis.These controls were developed to help lab managers to ensure that analytical error stays within acceptable limits.
AESQCs are unassayed ready to use reagents which should be handled in the same way as patient samples and can be used for monitoring the performance of ELISA and FARR assays.
Our AESQC controls are formulated to give positive reactions for the respective autoantibody and the lab should establish their own working ranges for each new lot depending from the test system and procedures being used.
AESQC Pool 3 can be used as function controls of AESKUBLOTS® Line Immuno Assays (LIA).
Antibodies:
aCL IgM, aPL IgM, B2GP1 IgG and IgM, DNA (ELISA & FARR)
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推荐了解“主细胞库”“工作细胞库”这两个名词。
① 在构建载体时,目的基因直接整合到细胞染色体组上,最好不要通过先瞬转在筛选稳定细胞株的这种方法,因为转染效率没有保证
② 高表达载体的构建,哺乳动物表达量一直是它自身的缺点,最好根据高表达载体定向的驯化细胞,提高蛋白表达量
③ 细胞的选择,筛选稳定细胞株我们常用的细胞是CHO,中国仓鼠卵巢细胞,由于CHO具有诸多的优点因此适合用于筛选稳定细胞株,而HEK293细胞则常用于瞬时转染
④ 后期的筛选,双抗预防污染,筛选细胞的时候抗生素浓度一定要做预实验,而且转染的时候不能有抗生素,关于细胞转染 稳定细胞系构建的相关理论
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
建立稳定细胞株,一般是根据不同基因载体中所含有的抗性标志选用相应的药物对靶...专注整体实验·服务生命科学已开展了数千个实验外包项目

