ESF921™InsectCellCultureMediumisacompleteserum-free,protein-freemediumdevelopedforrobustcellgrowth,proteinproductionandbaculovirusvectorproductionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFive™),andDrosophilaS2.ESF921containsL-GlutamineandKolliphor®P188(PluronicF68).
Description:
WidelyrecognizedastheindustrystandardforBEVSapplications,ExpressionSystemsinsectcellculturemediaaredesignedformaximumvirusandproteinexpression.Thisserum-free,protein-freeinsectcellculturemediumprovidesunmatchedcellgrowthandexpressionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFiveTM)andDrosophilaS2.
- Sf9celldensitiesof15x106cells/mlorgreater
- Produceshightiterbaculovirusvectoryieldsof5E8to3E9infectiousunits/ml
- Availablein1Lbottles,bulkpackagingandsingle-usebagsthateliminatetheneedtotransfermedia
- Complete,ready-to-use,containsL-glutamine
- Idealforscale-upmanufacturing
- Workswithawidevarietyofbaculovirusandhostcellplatforms
- Instockforimmediateshipment
- Adaptedcellsavailable
SuperiorGrowthandReproducibility
ExpressionSystems’ESF921TMInsectCellCultureMediumisdesignedformaximumexpressionforapplicationssuchas;genetherapydrugsubstance,virus-likeparticles,virusvectorsandproteinsofinterest.ESF921TMiswidelyrecognizedastheindustrystandardforthebaculovirusandinsecthostcellsystem.ThelinegraphpresentsreproducIBLegrowthcurvesforSf9cellculturesinninedifferentlotsofESF921TMmediummanufacturedoveraperiodof12months.

Sf9cellsfromamid-logphaseculturewereseededinto*9differentlotsofESF921TMatadensityof0.75×10,6/ml.Culturesweresampleddailytodetermineviablecellcount.
Specification:
| Mediatype | Ready-to-useserum-freeliquid,1X |
| Platformcompatibility | FunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms |
| Celllines | Spodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™)andDrosophilamelanogaster(S2) |
| Use-bydate | Oneyearfromdateofmanufacture |
RecommendedStorageCondition
2–8ºCebiomall.com
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要培养293A细胞,请教各位大神,配制好的完全培养基4度能放置多久?
HT培养基添加剂(HT Supplement)为粉剂,经照射灭菌。每个包装可配制成10ml浓缩液(50X),用于杂交瘤细胞的筛选。
各位大神你们好:
我们是医疗器械工厂,只需配制环境监测和水检测的培养基,但是根据2015版药典规定,1105,141页有一句
而在9203,289页
“商品化的成品培养基除了应附有处方和使用说明外,还应注明有效期、贮藏条件、适用性检查试验的质控菌和用途”。
是否可以理解为只要供应商提供以上信息,使用厂家就可以不用做培养基适用性试验了?
如果购买的商品化的成品培养基不做培养基适用性试验,那么医疗器械的微生物检验将大大减少工作量了。
琼脂,学名琼胶,英文名(agar),又名洋菜(agar-agar)、海东菜、冻粉、琼胶、石花胶、燕菜精、洋粉、寒天、大菜丝,是植物胶的一种,常用海产的麒麟菜、石花菜、江蓠等制成,为无色、无固定形状的固体,溶于热水。在食品工业中应用广泛,亦常用作细菌培养基。为什么叫琼脂,主要是用海南的麒麟菜或石花菜制作出来的。海南的简称就是琼。琼脂特性:琼脂的最有用特性是它的凝点和熔点之间的温度相差很大。它在水中需加热至95℃时才开始熔化,熔化后的溶液温度需降到40℃时才开始凝固,所以它是配制固体培养基的最好凝固剂。用琼脂配制的固体培养基,可用以进行高温培养而不熔化,在凝固之前接种时,也不致将培养物烫死。因此,琼脂是制备各种生物培养基中应用最广泛的一种凝固剂。琼脂的浓度,通常是液体培养基的1~1.5%。向左转|向右转

