Simpo-Mount is water-based mounting medium for immunohistology. It is used for the permanent preservation of the following chromogens or dyes. Simply apply drops of Simpo-Mount on the tissue section a liquid polymer coverslip will form. It does not need coverslip. This universal mounting media maintains the original stain intensity and characteristics. It demonstrates excellent optical clarity.
Compatible with:
1. Horseradish peroxidase chromogens: 1) Diaminobenzidine(DAB), 2) 3-amino, 9-ethylcarbazole (AEC), 3) 4-chloro-1-naphthol 2. Alkaline phosphatase chromogens: 1) 5-bromo, 4-chloro,indolylphosphate/ iodonitrotetrazolium(BCIP/INT), 2) 5-bromo, 4-chloro, 3-indolylphosphate/nitro- bluetetrazolium(BCIP/NBT) 3) Naphthol AS-MX phosphate/Fast Red TR salt. 3. Fluorescent specimen: 1) Fluorescein 2) Rhodamine 3) Texas Red 4) FluoroBlue
NOT Compatible with:
1. Fluorescent Specimen stained with: 1) Phycoerythrin, 2) Phycocyanin 3) Allophycocyanin. 4) 2. Conterstains: 1) Nuclear Fast Red 2) BC-50 3) Light Green 3. Tetramethylbenzidine (TMB) 4. Eosin
Recommended Protocol: 1. Recommended maximum section thickness is 5-8 microns. Thicker sections may produce air pockets 2. Must apply Simpo-Mount when tissue is wet. Air bubbles may be formed when tissue is dry 3. Rinse slide with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water and quickly wipe the back of the slide. 4. Invert dropper bottle to displace bubbles from the tip-end. 5. Squeeze out the first drop onto a paper towel to remove of air bubble on the tip. 6. Apply 3 drops of Simpo-Mount to cover the tissue section. DO NOT coverslip on top of the Simpo-Mount. 7. Rotate the slides to allow Simpo-Mount spread evenly to cover the tissue section. 8. Place slides horizontally in an oven at 40-50°C for at least 30 minutes or leave it at room temperature until slides are thoroughly dried. Slow dry at room temperature will help to eliminate the air bubbles. When use with fluorescent specimen, bake at 60-70°C for 20 minutes. 9. Hardened Simpo-Mount forms an impervious polymer barrier to organic solvent. 10. Allow sufficient time of drying slides before storage. 11. For maximum retention of fluorescence, slides should be kept in dark place at 4°C. 12. Do not use oil directly on the top of dried Simpo-Mount.
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用于酵母繁殖和筛选的培养基
SD是含有盐类、微量元素、维生素、氮源(不含氨基酸的酵母氮碱)和葡萄糖的合成基本培养基。
注意：氮源(不含氨基酸的酵母氮碱)是这种培养基用于筛选的关键，LB，YPD这种复合培养基因为用到了酵母提取物，蛋白胨，基本是氮源全营养的，肯定是不能用来做氮源筛选的
HT培养基添加剂(HT Supplement)为粉剂，经照射灭菌。每个包装可配制成10ml浓缩液（50X），用于杂交瘤细胞的筛选。
大家好，我是一个质粒小菜鸟，最近遇到个奇怪的事情，希望大家帮忙看看，究竟出了什么问题。
我构建了一个氨苄抗性的质粒，这个质粒在固体培养基里长出了单菌落，但是我挑菌在液体培养基里无法扩增出来。固体培养基和液体培养基一起配置的，氨苄浓度一致，唯一不同的是固体培养基里加入了琼脂。为了防止是氨苄的问题，我把这个固体培养基上的菌落挑划了新的板，依然能长出菌落。而同一次配置的液体培养基，也能扩增其他质粒，所以我实在想不出是什么其他问题，希望大家帮帮忙。
琼脂，学名琼胶，英文名（agar），又名洋菜(agar-agar）、海东菜、冻粉、琼胶、石花胶、燕菜精、洋粉、寒天、大菜丝，是植物胶的一种，常用海产的麒麟菜、石花菜、江蓠等制成，为无色、无固定形状的固体，溶于热水。在食品工业中应用广泛，亦常用作细菌培养基。为什么叫琼脂，主要是用海南的麒麟菜或石花菜制作出来的。海南的简称就是琼。琼脂特性：琼脂的最有用特性是它的凝点和熔点之间的温度相差很大。它在水中需加热至95℃时才开始熔化，熔化后的溶液温度需降到40℃时才开始凝固，所以它是配制固体培养基的最好凝固剂。用琼脂配制的固体培养基，可用以进行高温培养而不熔化，在凝固之前接种时，也不致将培养物烫死。因此，琼脂是制备各种生物培养基中应用最广泛的一种凝固剂。琼脂的浓度，通常是液体培养基的1～1.5%。向左转|向右转
