地高辛标记及检测试剂盒说明书
来自 : 蚂蚁淘
- MediaandSolutionrequiredforESCellCulture(BowtellLab)
- RoutineCulturingofESCells(BowtellLab)
- RoutineSplittingandfreezingofcells(PMCI)
- ESCellFreezingandThawing(NagyLab)
- ESCellCultureandManipulation(TheCancerCenteratWashingtonUniversity)
- CellCulturefromWholeMiceEmbryo(PMCI)ObtainEScellfrommiceembryoforculture
- ESCellCultureandManipulation(ChisholmLab)DetailedprotocolforEScellmanipulation,includingMedia,platingEScell,SplittingEScells,freezingEScells,transformationofEScells,expansionofEScellclonesandmore...
- ProductionofESCellChimerasbyAggregationwtihEight-CellStageEmbryos(NagyLab)ToproducecompletelyEScell-derivedembryosaclumpofEScellsissandwichedbetweentwotetraploidembryos.ForEScellaggregationchimeraswithdiploidembryos,oneembryoisaggregatedwithaclumpofEScells.Eveninthiscase,theEScellinternalizationisefficientenoughtoachieveahighlevelofEScellcontribution.
- ProductionofCompletelyESCell-DerivedFetusesbyAggragationwithTetraploidEmbryos(NagyLab)
- ES/MEFcellcultureandelectroporationoftargetingconstruct(GaryBrown)
- PreparationofMurineEmbryonicFibroblasts(GaryBrown)
- IsolationofPrimaryMouseEmbryoFibroblasts(PMCI)
- IsolationofPrimaryMouseEmbryoFibroblasts(BowtellLab)
- ElectroporationofESCellsandIsolationofH/RClones(BowtellLab)
- ElectroporationofESCell(NagyLab)
- KaryotypingESCells(BowtellLab)
- VasectomyofMaleMice(BowtellLab)
- MediaforEmbryoCultureandManipulation(BowtellLab)
- AggregationofESCellsandEightCellStageembryos(BowtellLab)
- BlastocystTransfer(BowtellLab)
- ES/MEFCellCultureandElectroporationofTargetingConstruct(GaryBrown)
- InVitroDifferentiationofESCellsinto:(NagyLab)
- CardiacMuscle
- NeuronalLineages
- CysticEmbryoidBodies
MitomycinCTreatmentofPMEFs(PMCI)GammaIrrADIationofPMEF"S(PMCI)PMEFFeederLayerConcentration(PMCI)FAQsforEmbyonicStemCellCulture(LTI)veryusefulinformationaboutmanyaspectsofEScellcultureNormaskiOptics(BowtellLab)
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