
Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestpossIBLetransfectionefficienciesandviABIlities.Lipofectamine®LTXReagentisanadvancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.Lipofectamine®LTXReagentoffers:
•Superiordelivery—transfectabroadrangeofcelltypes,includingprimary,challenging,andsensitivecells,withhighefficiency
•Superiorperformance—combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols—rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
Transfectprimarycellsefficiently
Primarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXReagent,includingprimaryneuralProgenitorcells.
Superiordeliveryintochallengingcells
WhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seefigure).Achievinghigh-leveltransgeneexpressionincellsthataretrADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
Broadcompatibilitywithcelllines
Inadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
Stabletransfection
Lipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXReagentthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,rapid,serum-compatibleprotocol
Lipofectamine®LTXReagentoffersastreamlinedprotocol—noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimpleoptimizationprotocolhelpsyougetthebestresultsquickly,soyou"llspendlesshands-ontime,andgetfasterresults.
Figures


ExamplesofLipofectamine®LTXtransfection

Lipofectamine®LTXtransfectionefficiency

Lipofectamine®LTXReagentcombineshightransfectionefficiencywithlowcytotoxicitysuperiortoFuGENE®6andFuGENE®HDtransfectionreagents

TransfectionoptimizationofHumanMesenchymalStemCells(hMCS)

Plasmidtransfection.

Lipofectamine®LTXinthepresenceofPLUS™Reagentisanexcellentchoicefortransfectionofhumancancercelllinesaswellasprimaryandstemcells.Thereagentenablesthedeliveryofhightransfectionperformancewithoutassociatedcytotoxicity

Lipofectamine®LTXReagent,withPlus™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Asimple,rapid,serum-compatibleprotocol

Lipofectamine®LTXtransfectionprotocol.

Lipofectamine®LTXReagent,withPLUS™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Lipofectamine®LTXTransfectionProcedureDetails

Lipofectamine®LTXTransfectionWorkflow

Specifications
CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
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SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR) |
Classification: | AnimalOrigin-Free |
TransfectionTechnique: | Lipid-BasedTransfection |
SerumCompatible: | Yes |
HighThroughputCompatibility: | HighThroughput-Compatible |
ProductLine: | Lipofectamine® |
ProductSize: | 1mL |
ShippingCondition: | WetIce |
Contents&storage
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可以用移液器将细胞吸出来并高速离心,沉淀重悬于PBS中洗涤,接着就可以裂解提取蛋白了。可以用超声,酶解等等,裂解后离心收集上清。
做的比较好的,一般都是上海地区的,你可以看下基尔顿生物。
我刚开始做转染,悬浮细胞,分别做过表达和敲减,看了很多文献,大都没有提及转染后是用转染的这同一批细胞同时做pcr,wb,cck8,凋亡,细胞周期;还是说这次转染只做pcr或wb,再转染一次做cck8或细胞周期。剩下的功能试验均同前,转染一次做一次?我养的是悬浮细胞,转染后做cck8这些功能试验前需要离心换液吗?跪谢解答!
细胞状态与密度;转染试剂的类型、用量;DNA的品质、用量;转染复合物的品质、作用细胞的时间长度。


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