YEPD(non-selection) Afterautoclavingaddglucoseto2%byadding100mlof20%solutiontoeachlitremedia. SYNTHETICMEDIA(selectivemedia) Afterautoclavingadd100mlof20%glucose(orgalactose)solutionand100mlof10XDropoutsolutiontoeachlitremedia. 10XDROPOUTSOLUTION *The10XDropoutsolutiondoesnotcontainHistidine,LeucineandTryptophan.Thesecomponentsareusedtomakeselectivemediatoselectforyeasttransformants. **Abetteroptionistopurchaseselectivedropoutaminoacidmix.CSM-His,-Leu,-Trp--BIO101(Cat.10427-100)andaddeitherHis,LeuorTrpasneeded.- 1%yeastextract - 2%peptone - 1.5%agar(ifneededforplates) - 0.67%yeastnitrogenbasewithoutaminoacids(Difco)0919153 - 1.5%agar(ifneededforplates).Alsoadd1pelletofNaOHperlitreofagartoavoidmushyplates. - L-Isoleucine300mg/L - L-Valine1500mg/L - L-Adeninehemisulfatesalt200mg/L - L-ArginineHCl200mg/L - L-LysineHCl300mg/L - L-Methionine200mg/L - L-Phenylalanine500mg/L - L-Threonine2000mg/L - L-Tyrosine300mg/L - L-Uracil200mg/L - L-HistidineHClmonohydrate200mg/L*SigmaH9511 - L-Leucine1000mg/L*SigmaL1512 - L-Tryptophan200mg/L*SigmaT0271
