最便宜的进口转染试剂，超过1800余篇世界一流科技论文的引用

产品介绍：
利用我们的创新和专有的脂质体共轭合成技术，LipoJet™转染试剂盒，运用了新型的含氟阳离子脂质体配方，同市场上的其他脂质体转染试剂有着显著的区别，其特点是转染效率高、毒性小。LipoJet™转染试剂盒是最强大的基因转导工具，适于几乎有所有体外基因转导的应用，包括质粒DNA、siRNA和shRNA转染和DNA/siRNA共转等。

试剂盒内容：
-LipoJet™ 试剂，1.0ml，足够用1000次DNA的转染(24孔板中0.5ugDNA/孔)足够用1000次siRNA转染(24孔板中10nMsiRNA/孔)。
-LipoJet™转染缓冲液(5X)，按照最大化的转染效率配制，8.0ml浓缩液就可以制成40ml的工作液体。

产品特点：
- 适用于广泛的哺乳动物细胞
- 极高效率地转染DNA，siRNA和DNA/siRNA共转染
-10nMsiRNA能达到95%的沉默效应
- 无血清和抗生素干扰
- 简单易用的操作步骤
- 适用于高通量的应用
-细胞毒性最低

储存条件 ：
4⁰C储存。若储藏合适，产品的稳定性能保持12个月以上。

LipoJet™体外转染试剂具有极广谱的转染活性。

以下图例表明LipoJet™体外转染试剂盒出色的DNA和siRNA转染效率。

AefficiencycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHela,Cos-7,NIH-3T3,CHOandHEK293(leftpanel)andCos-7(rightpanel).  pEGFP-N3cDNA(0.5µg/wellof24-wellplate)wastransfectedintodifferentmammaliancellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. ThetransfectionefficiencywereanalyzedviaFACS48hoursposttransfection.


AtoxicitycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHelacells.  pEGFP-N3cDNA(1.0µg/wellof24-wellplate)wastransfectedtoHelacellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. TheMTTassay(rightpanel)andphasecontrastimaging(leftpanel)wereusedtoanalyzethecellviability48hoursposttransfection. 


AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHEK293Tcell. pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedinto293Tcellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA (µl/µg) ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA (µl/µg) ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.


AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHelacell. pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedintoHelacellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA (µl/µg) ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA (µl/µg) ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.




LipoJet™试剂介导的DNA/siRNA共转染表现出色的基因沉默效应。分别由LipoJet™试剂转染GFP的cDNA(左图,0.25µg/孔，24孔板)作为对照和共转染GFP的cDNA(0.25µg/孔，24孔板)/GFP的siRNA(终浓度10nM，右图)至HEK293细胞(上图),Hela细胞(中图)和SaoS-2细胞(下图)。转染24小时后，使用尼康Eclipse荧光显微镜检查GFP荧光。结果显示，GFP的cDNA和GFP的siRNA共转染导致GFP的表达被显著抑制。


ExceptionalDNA/siRNAco-transfectionefficiencyonHUVEC. Co-transfectionofmCherrycDNA(0.10µgperwellof24-wellplate)andFITCconjugatedsiRNA(final30nMperwellof24-wellplate)toHUVECwithLipoJet™reagentgaveriseto60%mCherry+(phasecontrastoverlappedwithmCherryimaging,leftpanel)andnearly100%FITC-siRNA+(phasecontrastoverlappedwithFITCimaging,rightpanel)HUVEC24hoursaftertransfection. ThepicturesweregivenfromDr.PanKongofUSCascourtesy.  



ExcellentsilencingofendogenouslyexpressedKIF11(alsoknownasEG5)inHEK293(upperpanel)andHela(lowerpanel)cellswithLipoJet™reagentat10nMEG5siRNA. KIF11(alsoknownasEG5)encodesamotorproteinthatbelongstothekinesin-likeproteinfamilyinvolvedinchromosomepositioningandbipolarspindleformationduringcellmitosis.AreductioninKIF11levelscausesmitoticarrest.LipoJet™reagenteffectivelydeliversEG5siRNA(final10nM)toHEK293andHelacells,leadingtomorethan80%of"round-up"phenotypeofHEK293andHelacells24hposttransfectionovernegativecontrol(final10nMwithshamEG5siRNA).Thephenotypeof"rounded-up"HEK293andHelacellswerevisualized24hposttransfectionwithaNikonmicroscope. 



LipoJet™转染试剂介导的基因沉默可显著地抑制HEK293细胞(上图)和Hela(下图)内源型EG5的表达。KIF11(也称为EG5)编码了一个启动蛋白，这启动蛋白属于驱动蛋白家族，参与染色体定位和细胞有丝分裂时两端的纺锤体的形成。EG5水平降低会阻碍有丝分裂。LipoJet™试剂能有效的将EG5siRNA(终浓度10nM)导入到HEK293和Hela细胞中。转染24小时后，参照阴性对照(终浓度是10nM+假EG5siRNA)，LipoJet™介导的EG5siRNA的导入显著地抑制了细胞有丝分裂，导致了大于80%的细胞出现圆顶表型。

转染试剂操作步骤及其使用指南： 
- LipoJet™试剂DNA和siRNA转染操作步骤 
- 简易DNA转染操作步骤(适合熟练操作者 
- 简易siRNA转染操作步骤(适合熟练操作者) 
- LipoJet™试剂DNA/siRNA共转染操作步骤 
- 注意事项及转染窍门 



用户评价：

Itriedtheplasmid/siRNAco-transfectionusingtheLipoJetsampleinEAhy926cellwhichisaHUVECcellline.Theresultsareprettygood,atleastforthetransfectionefficiency.Fortheplasmidtransfection,48hourslater,around60%cellaretransduced.ForsiRNA,theefficiencyisalmost100%.WeareorderingmoreLipoJetreagenttousefromnow.

-----Dr.PanKong,UniversityofSouthernCalifornia

Wegot>90%efficiencywithLipoJetvs.80%withX-tremeGENE9on293Tcell.Definitelywillordermore....

-----Dr.NuoYangfromRoswellParkCancerInstitute

HerearetheresultsfromourpreliminaryexperimentswithLipoJet.Weareverysatisfiedwithitsefficiency.Unfortunately,wewereoutofFugene6/HDandwereunabletotestthemsidebyside,butbasedonpreviousexperience,wedobelievethatyourproductworkedwithbetterefficiency.

------Dr.AlisonMcKelveyfromUniversityofPittsburgh

IamhappytosaythatIhadgreatsuccesswithyourtransfectionreagentsonOKF6/TERT2humankeratinocytes.IwillputtogetherthedataonceIhavecompletedmyanalysis.IreallyliketheLipoJet.MycellofinterestdidnotdoverywellintheGenMutealthoughmycontrolcellsdid.WewanttogoaheadwithorderingASAP.Ialsohaveacouponfor10%foraPOorder.

-----Dr.MichelleSimpson-AbelsonfromUPMC

WedidindeedtestthemsidebysidewithourhomemadeCaPhos.Theresultsaregreat, LipoJetandCalFectinbothdidextremelywell.LipoJetbeingthebetterofthetwo.Iwillsendyoutheresultswhen Igetthem, Iamcurrentlyoutofthelabbutwewillbeorderingmoreforcertain.Thankyouforsendingthosesamples.

------Dr.AhmedHassib,CornellUniversity

FortheLipoJet,IonlycompareditwithLipoLTXinHelacells.TheLipoJetgaveamuchhigherefficiency(~35%betterthanLipoLTX)24hoursaftertransfection.WewillordersomeLipoJet.

-----AbetatesterfromUSC