Description
GenJet™DNA InVitro TranfectionReagentfor BHK-21cellispre-optimizedfortransfectingBHK-21cells. BHK-21cellsisoneofthemostcommonlyusedcellsforvaccineproduction.Whenusedforthisapplication,usuallyacellstrainadaptedtogrowinsuspensionisused. Refertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyon BHK-21 cells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates.
SummaryofOptimalTransfectionConditions:ConfluenceonthedayoftransfectionCellcultureconditionsGenJet™(µl):DNA(µg)RatioDiluentforDNAandTransfectionReagentIncubationTimetoFormGenJet™/DNAComplexPresenceofSerum/AntibioticsduringTransfectionChangeMediumAfterTransfectionMaximalEfficiency TransfectionResults:ReporterGenePlasmidEfficiency(GFP%) | 80%DMEMwith4.5g/Lglucose,10%FBS3:1Serum-freeDMEMwith4.5g/Lglucose15minutesatRTYesYes,5HoursAfterTransfection48hoursEGFPpEGFP-N3(CMVpromoter) 85% |
StorageConditionStoreat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger
DataSheet.pdf
