AQUASOL焊接气囊IPURGEABLD2(2"),ABLD4(4"),ABLD6 ...
| 货号: | SL100489-PAC |
| 保存条件: | 4°C |
| 供应商: | 济南美中清水湾生物科技有限公司 |
| 数量: | 现货大量 |
| 英文名: | GenJet™InVitroDNATranfectionReagentforM-PACCells |
| 保质期: | 一年 |
| 规格: | 毫升/支 |
Description
GenJet™DNA InVitro TranfectionReagentforM-PACispre-optimizedfortransfectingM-PAC(alsocelledmPAC)cells. M-PACismoderatelyimpregnatedpancreasacinarunitcells,also oneofhard-to-transfectcellline.
RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonM-PACcells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor50to100transfectionsin6wellplates.
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger
DataSheet
GenJet™DNA InVitro TranfectionReagentforM-PACispre-optimizedfortransfectingM-PAC(alsocelledmPAC)cells. M-PACismoderatelyimpregnatedpancreasacinarunitcells,also oneofhard-to-transfectcellline.
RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonM-PACcells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor50to100transfectionsin6wellplates.
| SummaryofOptimalTransfectionConditions: Confluenceonthedayoftransfection Cellcultureconditions GenJet™(µl):DNA(µg)Ratio DiluentforDNAandTransfectionReagent IncubationTimetoFormGenJet™/DNAComplex PresenceofSerum/AntibioticsduringTransfection ChangeMediumAfterTransfection MaximalEfficiency TransfectionResults: ReporterGene Plasmid Efficiency(GFP%) | 90% DMEMwith4.5g/Lglucose,10%FBS 3:1 Serum-freeDMEMwith4.5g/Lglucose 15minutesatRT Yes Yes,~5HoursAfterTransfection 48hours EGFP pEGFP-N3(CMVpromoter) 60% |
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger
DataSheet
温馨提示:不可用于临床治疗。
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发布于 : 2021-08-30
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