ORDERINGINFORMATION
Catalogno.:56582(cloneS231B-34)
Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.
BACKGROUND
Leucine-richrepeatkinase2(LRRK2),alsoknownasdardarin,isanenzymethatinhumansisencodedbythePARK8gene.Theproteinispresentlargelyinthecytoplasmbutalsoassociateswiththemitochondrialoutermembrane.MutationsinthisgenehavebeenassociatedwithParkinson'sdiseasetype8andCrohn’sdiseasewhichsuggeststhatthesetwodiseasesshareacommonpathway.ExpressionofmutantLRRK2inducedapoptoticcelldeathinneuroblastomacellsandinmousecorticalneurons.
SPECIFICATIONSUMMARY
Antigen:Fusionproteincorrespondingtoaa841-960ofhumanLRRK2.Thissequenceis81%identicalinmouse,80%identicalinrat,and<30%identicalinLRRK1.
Accessionnos:NP_940980,Q5S007
GeneID:120892
HostSpecies:Mouse
AntibodyClass:IgG2a
Specificity:ThisantibodyrecognizeshumanandratLRRK2.
APPLICATIONS
Immunoblotting:useat1-5ug/ml.Abandof
>200kDaisdetected.
Immunofluorescence:useat10ug/ml.
DetectionofLRRK2inratbrainlysatewith
#56582at5ug/ml.
DetectionofLRRK2inneuroblastomacelllineSK-N-BEwith#56582at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)LRRK2stain.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisproductisstableforatleastone(1)yearat-20oC.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构,同步动态侦测细胞有氧呼吸,糖酵解OCR/ECA(总或乳酸ECA);
i.过氧敏感荧光素(或pH敏感荧光素),Ex340-380/535/Em630-680nm,实时测量线粒体/胞内/胞外重要代谢指标,荧光素为非结构性结合可逆转改变;
ii.可同时或单个测量OCR/ECA或其他参数,不会做成浪费,可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口,可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用