productdatasheet
CatalogNo.:56474(clone1D4)Size:100uginPBS,pH7.4;50%
glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
BACKGROUNDRhodopsiniscomprisedoftheproteinopsinandareversIBLycovalentlyboundcofactor,retinal.Opsin,madeupofsevenmembrane-embeddedalphahelices,bindsretinal,aphotoreactivechromophore,inacentralpocket.Inadditiontobeingthepigmentoftheretinathatisresponsiblefortheformationofphotoreceptorcells,rhodopsinconveysinformationstoredinthechromophoretothesurfaceofthemoleculeuponlightabsorption.Mutationsintherhodopsingeneleadtoretinitispigmentosa.
ThisantibodyrecognizestheC-terminalaaTETSQVAPA(COOH)ofnativeandrecombinantvertebraterhodopsin.
Immunoblotting:useat1-10ug/ml.Abandof~40kDaisdetected.
Immunohistochemistry/immunocyto-chemistry:useat1-10ug/mlImmunoprecipitation:useat1-2ugper100-500ugofprotein.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Rateyelysate
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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SanDiego,CA92127
TollFree800.929.2114
Phone858.675.2405
Fax858.592.1509
info@qedbio.com
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细胞质中有各种细胞器,细胞内的化学反应大多都是在细胞质中进行,因此是代谢中心.
细胞核中有DNA,是遗传信息库,是细胞代谢和遗传的控制中心.
解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
不破坏细胞结构,同步动态侦测细胞有氧呼吸,糖酵解OCR/ECA(总或乳酸ECA);
i.过氧敏感荧光素(或pH敏感荧光素),Ex340-380/535/Em630-680nm,实时测量线粒体/胞内/胞外重要代谢指标,荧光素为非结构性结合可逆转改变;
ii.可同时或单个测量OCR/ECA或其他参数,不会做成浪费,可采用时间分辨荧光技术可加强系统信噪比
iii.通过两个加药口,可对检测细胞加入适当抑制剂/刺激实时监测细胞对不同毒素作用