Source | M.W. | CAS No. | |||
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Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
Reconstitution | Before reconstitution, a brief spin is recommended todrive down any material dislodged from the bottom of the tube. The lyophilized protein should bereconstituted in sterile H2O to a desired concentration. | ||||
Stability | The lyophilized protein is stable for at least oneyear if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C,but should be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The EC50 ranges from 5 - 20 ng/ml in the presence of 10 ng/mL human WNT-3a. | ||||
Country of Origin | USA |
R-spondin-1 is a natural enhancer of the canonicalWNT pathway. When used together with WNT proteins that activate thebeta-catenin pathway, R-spondin-1 enhances the activity of canonical WNTproteins by binding to LGR5 and LGR4 (refs.)Injection of recombinant RSpondin-1 intomouse causes activation of the βcatenin pathway and proliferation of intestinal crypt cells, which forms the basis for aclinical trial in amelioration of chemotherapy-induced colitis.Thisproduct is the full-length R-Spondin-1 fused at its C-terminus to the Fc domainof human IgG1. This fusion increases thestability of the protein in vitro and in vivo without compromising itsbiological activity. StemRD’s R-Spondin-1 Fc fusion protein isproduced in human 293 cells as a secreted protein and purified by protein Achromatography.Refs: de Lau, et al, LGR5 homologuesassociate with Wnt receptors and mediate R-spondin signaling. Nature. 2011 July 4; 476: 293; Carmon, et al, R-spondins function as ligandsfor the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling.PNAS. 2011 Jul 12; 108: 11452.
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CD11C:树突细胞,单核细胞,巨噬细胞,中性粒细胞
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友
可以用CCR3的抗体标记其他细胞,再反推中性粒细胞所占的比例吗?肺泡灌洗液中主要有嗜酸性粒细胞,淋巴细胞,中性粒细胞和巨噬细胞。
(2)荧光标记法 : 使用二乙酸荧光素(FDP)、碘化丙啶(PI)或异硫氰酸荧光素钠标记的荧光染料与细胞共孵育,用流式细胞仪检测荧光染色阳性细胞的比率。此法其实是(1)法的“荧光”版,但其在灵敏性和准确性方面明显要优于后者。
(3)硝酸镧(La)示踪法: 在正常的生物组织中镧微粒可沉积于细胞间隙,但不能穿过具有1~ 2nm 微小间隙的细胞膜性结构(包括细胞膜和细胞器膜),也不能穿过细胞间的紧密连接。在膜性结构通透性增高时, 镧微粒则可进入细胞、细胞器和紧密连接内, 并在电镜下显示, 镧盐标记技术被认为是一种有效的监测细胞膜通透性变化的标记技术。
(4)LDH释放法: 在正常情况下,细胞内大分子物质LDH 是不能通过细胞膜的, 但在细胞膜受损伤而通透性增加时,可通过受损的细胞膜释放出来。LDH 能较好地反映细胞膜损伤程度。类似的还有检测细胞外K+的漏出率等。