
Product Name | SensoLyte ® 490 MMP - 13 Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-71135 |
US$ | $663 |
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma. The SensoLyte® 490 MMP-13 Assay Kit is a complete assay system designed to continuously analyze MMP-13 activities or to screen MMP-13 inhibitors by using a fluorogenic MMP-13 substrate. The assays are performed in a convenient 96-well or 384-well microplate format. The kit provides speed, convenience and accuracy in analyzing MMP-13 activity and screening MMP-13 inhibitors.Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-13 activity, please choose the SensoLyte® Plus MMP-13 Assay Kit, Cat# 72019. Kit size: 500 assays | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
Product Citations | Chao, YH. et al. (2010). The cross-talk between transforming growth factor-beta1 and ultrasound stimulation during mechanotransduction of rat tenocytes. Connective Tissue Res doi:10.3109/03008207.2010.525673. |
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Thy-1、c-kit散在分布于HB组织中,主要集中在汇管区,而正常肝脏组织中不表达;CD34及SCF在HB中的表达明显高于正常肝脏组织(P0.05),其中CD34主要在血管内皮系统中分布,SCF主要表达在汇管区;CD56表达于成簇的神经纤维组织中,在HB及正常肝脏组织中表达差异无统计学意义(P0.05)。结论不同干细胞相关表面标志物分布于HB组织中,并且集中表达在特定区域。表达Thy-1/c-kit阳性细胞可能对HB的发生起一定作用。
CD11C:树突细胞,单核细胞,巨噬细胞,中性粒细胞
比如你用的CD1a-FITC(如果是鼠单抗IgG1,那对照抗体就要用相同物种的非特异性IgG1-FITC)。注意浓度要相同。一般提供抗体的公司BD,santa cruz等有提供的。其他就按照说明书的推荐浓度和孵育时间。
1,逆转录病毒将GFP基因导入干细胞,这种办法在导入基因时好像比较麻烦,但是检测好像比较简单。
2,将雄性干细胞植入雌性动物,追踪Y染色体,即SRY基因,但是看文献上说SRY追踪的FISH技术复杂,费用高,但是还没有看到具体的技术步骤,也不知道具体要多少费用。
请多指教
有几个疑问
1:荧光标记到细胞是标记到细胞表面还是细胞质内?
2:荧光应该随着细胞的分化和增殖逐渐消失?是不是分化增殖越快,荧光消失速度越快?
3:有哪些容易操作,成本便宜的荧光物质?
谢谢各位战友

