Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- Product No.PSB949Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
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- Multi-species
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- Reagent Contents
Recombinant Transforming Growth Factor Beta 3 (TGFb3) 50ug Standard Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) 200ug Detection Ab Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) 500ug Capture Ab - ApplicationsELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
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- FOBUS$ 1078 For more details, please contact local distributors!US$ 1796 For more details, please contact local distributors!US$ 3233 For more details, please contact local distributors!US$ 6286 For more details, please contact local distributors!US$ 10776 For more details, please contact local distributors!
MATCHING TESTS of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
USAGE of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
The concentration of protein/peptide and antibodies used in different immunoassays need to be determined by users according to their experiments.
STORAGE of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceStandard DiluentCoating BufferDiluent Buffer for AntibodyAntibody Pairs Support Pack 1Antibody Pairs Support Pack 2Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB949Hu02 | Recombinant Transforming Growth Factor Beta 3 (TGFb3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB949Hu01 | Recombinant Transforming Growth Factor Beta 3 (TGFb3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB949Hu01 | Active Transforming Growth Factor Beta 3 (TGFb3) | Cell culture; Activity Assays. |
| PAB949Hu01 | Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| PAB949Hu02 | Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| LAB949Hu71 | Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC. |
| MAB949Hu22 | Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| MAB949Hu24 | Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| SEB949Hu | ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB949Hu | CLIA Kit for Transforming Growth Factor Beta 3 (TGFb3) | Chemiluminescent immunoassay for Antigen Detection. |
| PSB949Hu01 | Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB949Hu01 | ELISA Kit DIY Materials for Transforming Growth Factor Beta 3 (TGFb3) | Main materials for "Do It (ELISA Kit) Yourself". |
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求采纳为满意回答。
在缓冲溶液中加入少量强酸或强碱,其溶液pH值变化不大,但若加入酸,碱的量多时,缓冲溶液就失去了它的缓冲作用。这说明它的缓冲能力是有一定限度的。
缓冲溶液的缓冲能力与组成缓冲溶液的组分浓度有关。0.1mol·L-1HAc和0.1mol· L-1NaAc组成的缓冲溶液,比0.01mol·L-1HAc和0.01mol·L-1NaAc的缓冲溶液缓冲能力大。关于这一点通过计算便可证实。但缓冲溶液组分的浓度不能太大,否则,不能忽视离子间的作用。
组成缓冲溶液的两组分的比值不为1∶1时,缓冲作用减小,缓冲能力降低,当c(盐)/c(酸)为1∶1时△pH最小,缓冲能力大。不论对于酸或碱都有较大的缓冲作用。缓冲溶液的pH值可用下式计算:
此时缓冲能力大。缓冲组分的比值离1∶1愈远,缓冲能力愈小,甚至不能起缓冲作用。对于任何缓冲体系,存在有效缓冲范围,这个范围大致在pKaφ(或pKbφ)两侧各一个pH单位之内。
弱酸及其盐(弱酸及其共轭碱)体系pH=pKaφ±1
弱碱及其盐(弱碱及其共轭酸)体系pOH=pKbφ±1
例如HAc的pKaφ为4.76,所以用HAc和NaAc适宜于配制pH为3.76~5.76的缓冲溶液,在这个范围内有较大的缓冲作用。配制pH=4.76的缓冲溶液时缓冲能力最大,此时(c(HAc)/c(NaAc)=1。
制备
为了配制一定pH的缓冲溶液,首先选定一个弱酸,它的pKaφ尽可能接近所需配制的缓冲溶液的pH值,然后计算酸与碱的浓度比,根据此浓度比便可配制所需缓冲溶液。
以上主要以弱酸及其盐组成的缓冲溶液为例说明它的作用原理、pH计算和配制方法。对于弱碱及其盐组成的缓冲溶液可采用相同的方法。
缓冲溶液在物质分离和成分分析等方面应用广泛,如鉴定Mg2+ 离子时,可用下面的反应:
白色磷酸铵镁沉淀溶于酸,故反应需在碱性溶液中进行,但碱性太强,可能生成白色Mg(OH)2沉淀,所以反应的pH值需控制在一定范围内,因此利用NH3·H2O和NH4Cl组成的缓冲溶液,保持溶液的pH值条件下,进行上述反应。
常用缓冲液配制
枸橼酸-磷酸氢二钠
甲液:取枸橼酸21g或无水枸橼酸19.2g,加水使溶解成1000ml,置冰箱内保存。
乙液:取磷酸氢二钠71.63g,加水使溶解成1000ml。
取上述甲液61.45ml与乙液38.55ml,混合,摇匀,即得。
氨-氯化铵缓冲液
取氯化铵1.07g,加水使溶解成100ml, 再加稀氨溶液(1→30)调节pH值至8.0,即得。
氨-氯化铵缓冲液
取氯化铵5.4g,加水20ml溶解后,加浓氨溶液35ml,再加水稀释至100ml,即得。
醋酸-醋酸钠缓冲液
取无水醋酸钠20g,加水300ml溶解后,加溴酚蓝指示液1ml及冰醋酸60~80ml,至溶液从蓝色转变为纯绿色,再加水稀释至1000ml,即得。
醋酸-醋酸钠缓冲液
取醋酸钠18g,加冰醋酸9.8ml,再加水稀释至1000ml,即得。
醋酸-醋酸钠缓冲液
取醋酸钠54.6g,加1mol/L醋酸溶液20ml溶解后,加水稀释至500ml,即得。
醋酸-醋酸铵缓冲液
取醋酸铵7.7g,加水50ml溶解后,加冰醋酸6ml与适量的水使成100ml,即得。
醋酸-醋酸铵缓冲液
取醋酸铵77g,加水约200ml使溶解,加冰醋酸57ml,再加水至1000ml,即得。
醋酸-醋酸铵缓冲液
取醋酸铵100g,加水300ml使溶解,加冰醋酸7ml,摇匀,即得。
磷酸盐缓冲液
取0.2mol/L磷酸二氢钾溶液250ml,加0.2mol/L氢氧化钠溶液118ml,用水稀释至1000ml,即得。
当往某些溶液中加入一定量的酸和碱时,有阻碍溶液pH变化的作用,称为缓冲作用,这样的溶液叫做缓冲溶液。弱酸及其盐的混合溶液(如HOAc与NaOAc),弱碱及其盐的混合溶液(如NH3·H2O与NH4Cl)等都是缓冲溶液。
参考资料:http://baike.baidu.com/view/901429.htm


