Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- Product No.PSB949Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
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- Human
- Mouse
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- Caprine
- Ovine
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- Canine
- Others
- Multi-species
- Pan-species
- Reagent Contents
Recombinant Transforming Growth Factor Beta 3 (TGFb3) 50ug Standard Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) 200ug Detection Ab Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) 500ug Capture Ab - ApplicationsELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
- Downloadn/a
- FOBUS$ 1078 For more details, please contact local distributors!US$ 1796 For more details, please contact local distributors!US$ 3233 For more details, please contact local distributors!US$ 6286 For more details, please contact local distributors!US$ 10776 For more details, please contact local distributors!
MATCHING TESTS of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
USAGE of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
The concentration of protein/peptide and antibodies used in different immunoassays need to be determined by users according to their experiments.
STORAGE of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceStandard DiluentCoating BufferDiluent Buffer for AntibodyAntibody Pairs Support Pack 1Antibody Pairs Support Pack 2Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB949Hu02 | Recombinant Transforming Growth Factor Beta 3 (TGFb3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB949Hu01 | Recombinant Transforming Growth Factor Beta 3 (TGFb3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB949Hu01 | Active Transforming Growth Factor Beta 3 (TGFb3) | Cell culture; Activity Assays. |
| PAB949Hu01 | Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| PAB949Hu02 | Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| LAB949Hu71 | Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC. |
| MAB949Hu22 | Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| MAB949Hu24 | Monoclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3) | WB; IHC; ICC; IP. |
| SEB949Hu | ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB949Hu | CLIA Kit for Transforming Growth Factor Beta 3 (TGFb3) | Chemiluminescent immunoassay for Antigen Detection. |
| PSB949Hu01 | Antibody Pair for Transforming Growth Factor Beta 3 (TGFb3) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB949Hu01 | ELISA Kit DIY Materials for Transforming Growth Factor Beta 3 (TGFb3) | Main materials for "Do It (ELISA Kit) Yourself". |
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求采纳为满意回答。
碳酸氢钠溶液是一种co2缓冲液,当瓶内二氧化碳量减少时,碳酸氢钠溶液释放co2,反之吸收co2.因此它能保持瓶内二氧化碳量大致不变.
1 mmol/LEDTA(pH 8.0)
因为含有以上两种物质,所以称为TE。
配制分三步:
1)1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
2)0.5 M EDTA(pH 8.0)50 ml的配制:称取EDTA-Na2·2H2O 9.306 g,加超纯水35 ml,剧烈搅拌,用约1 g NaOH颗粒调pH至8.0,定容至50 ml。(EDTA二钠盐需加入NaOH将pH调至接近8.0时,才会溶解。)
3)1×TE(10 mM Tris-HCl,pH 8.0;1 mM EDTA,pH 8.0)的配制:
作用:
TE缓冲液是弱碱性,对DNA的碱基有保护性,(DNA在它是的稳定性较好,不易破坏其完整性或产生开环及断裂),包括提取好的DNA也要放在TE缓冲液是保存. 10mMTris-Hcl,pH有7.47.68.0三种。
EDTA调到8.0是为了更好溶解,其他只要调到相应pH就可以。Tris在7-8附近缓冲能力很强,所以加8.0的EDTA下去后,不会改变pH。
1、组成成分:
A、1×TE缓冲液:10mmol/LTris.Cl;1mmol/LEDTA,pH8.0。
B、1×TAE缓冲液:40mmol/LTris-乙酸;2mmol/LEDTA,pH8.0。
C、1×TBE缓冲液:45mmol/LTris-硼酸;1mmol/LEDTA,pH8.0。
2、用途:
A、TE缓冲液:一般用作溶解剂或保持剂,常用于溶解DNA,能稳定储存DNA。
B、TAE缓冲液:生物学中使用最广泛的核酸电泳缓冲液,主要用于DNA的琼脂糖凝胶电泳。
C、TBE缓冲液:生物学中常用的核酸电泳缓冲液,主要用于DNA的琼脂糖凝胶电泳。
3、TAE和TBE缓冲液的选择:
TAE和TBE缓冲液都可以用于DNA的琼脂糖凝胶电泳,两者各有利弊,应根据实际情况选择不同的缓冲液。
4、注意:
TBE浓溶液长时间存放后会形成沉淀物,出现沉淀后应予以废弃。
TBST中含有Tris-Hcl,NaCl,Tween20这三种物质,是做WESTERNBLOT中常用的一种缓冲液。
TBST缓冲液的配制
1000ml×TBST的配置
先称量NaCl40g,倒入烧杯中,加DDW蒸馏水400ml,再称量NaCl47.6g,倒入刚才的那个烧杯中(PS:由于NaCl的量太多,一次称量不方便,所以分两次称量,且易于溶解)。往烧杯中加入Tris—HCl缓冲液100ml,最后加(吐温20)5ml,转入1000ml容量瓶中,在定容,转移即可。
TBST缓冲液的应用:
1.主要用于免疫组化和原位杂交,酶联免疫等实验中,清洗免疫印。
2.迹膜;
注意事项:
1.TBST缓冲液,PH7.2-7.5;
2.颜色为无色透明液体;
3.为了您的安全和健康,请穿实验服并戴防护手套操作;


